Isolated from the plant Salvia sclarea (clary sage). Originally thought to be synthesized in one step from geranylgeranyl diphosphate it is now known to require two enzymes, EC 126.96.36.199, copal-8-ol diphosphate synthase and EC 188.8.131.52, sclareol synthase. Sclareol is used in perfumery.
enzymes labda-13-en-8-ol diphosphate synthase and sclareol synthase are two monofunctional diterpene synthases which, together, produce the diterpenoid specialized metabolite sclareol in a two-step process. Labda-13-en-8-ol diphosphate synthase produces labda-13-en-8-ol diphosphate as major product from geranylgeranyl diphosphate with some minor quantities of its non-hydroxylated analogue, (9 S, 10 S)-copalyl diphosphate. Sclareol synthase then transformes these intermediates into sclareol and manool, respectively