Information on EC 4.2.3.141 - sclareol synthase

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The expected taxonomic range for this enzyme is: Salvia sclarea

EC NUMBER
COMMENTARY hide
4.2.3.141
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RECOMMENDED NAME
GeneOntology No.
sclareol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate + H2O = sclareol + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate-lyase (sclareol forming)
Isolated from the plant Salvia sclarea (clary sage). Originally thought to be synthesized in one step from geranylgeranyl diphosphate it is now known to require two enzymes, EC 4.2.1.133, copal-8-ol diphosphate synthase and EC 4.2.3.141, sclareol synthase. Sclareol is used in perfumery.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzymes labda-13-en-8-ol diphosphate synthase and sclareol synthase are two monofunctional diterpene synthases which, together, produce the diterpenoid specialized metabolite sclareol in a two-step process. Labda-13-en-8-ol diphosphate synthase produces labda-13-en-8-ol diphosphate as major product from geranylgeranyl diphosphate with some minor quantities of its non-hydroxylated analogue, (9 S, 10 S)-copalyl diphosphate. Sclareol synthase then transformes these intermediates into sclareol and manool, respectively
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(9S,10S)-copalyl diphosphate + H2O
manool + diphosphate
show the reaction diagram
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?
labda-13-en-8-ol diphosphate + H2O
sclareol + diphosphate
show the reaction diagram
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?
additional information
?
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
; enzyme possesses an N-terminal signal sequence that actively targets sclareol synthase to the chloroplast
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61000
x * 61000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 61000, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli and Saccharomyces cerevisiae
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
synthesis of sclareol by co-expression of producing labda-13-en-8-ol diphosphate as major product and manool synthase in Saccharomyces cerevisiae