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Information on EC 4.2.3.140 - cis-abienol synthase and Organism(s) Abies balsamea and UniProt Accession H8ZM73

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.140 cis-abienol synthase
IUBMB Comments
Isolated from the plants Abies balsamea (balsam fir) and Nicotiana tabacum (tobacco) .
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This record set is specific for:
Abies balsamea
UNIPROT: H8ZM73
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Word Map
The taxonomic range for the selected organisms is: Abies balsamea
The enzyme appears in selected viruses and cellular organisms
Synonyms
ntabs, abcas, cis-abienol synthase, z-abienol synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cis-abienol synthase
-
Z-abienol synthase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(13E)-8alpha-hydroxylabd-13-en-15-yl-diphosphate-lyase (cis-abienol forming)
Isolated from the plants Abies balsamea (balsam fir) [1] and Nicotiana tabacum (tobacco) [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate
cis-abienol + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate
cis-abienol + diphosphate
show the reaction diagram
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAS_ABIBA
867
0
99539
Swiss-Prot
Chloroplast (Reliability: 4)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modeling based on PDB entry 3p5pA. The active site residues Asp-48, Leu617, Phe696, and Gly723 are potentially important for the specificity
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D402A/D404A
D621A
monofunctional class I protein variant, mutant produces trace amounts of copalyl diphosphate plus labda-13-en-8,15-diol
additional information
lab-scale cultivation and pilot-scale cultivation setup of transgenic cell wall-deficient Chlamydomonas reinhardtii strain TN72-TPS4 recombinantly expressing enzyme TPS4, for establishment of industrial production of abietol, method evaluation, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene TPS4, recombinant expression in a cell wall-deficient Chlamydomonas reinhardtii strain TN72-TPS4 in chloroplasts, pilot-scale cultivation setup, method overview
recombinantly expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zerbe, P.; Chiang, A.; Yuen, M.; Hamberger, B.; Hamberger, B.; Draper, J.A.; Britton, R.; Bohlmann, J.
Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production
J. Biol. Chem.
287
12121-12131
2012
Abies balsamea (H8ZM73), Abies balsamea
Manually annotated by BRENDA team
Zedler, J.A.; Gangl, D.; Guerra, T.; Santos, E.; Verdelho, V.V.; Robinson, C.
Pilot-scale cultivation of wall-deficient transgenic Chlamydomonas reinhardtii strains expressing recombinant proteins in the chloroplast
Appl. Microbiol. Biotechnol.
100
7061-7070
2016
Abies balsamea (H8ZM73)
Manually annotated by BRENDA team