Information on EC 4.2.3.138 - (+)-epi-alpha-bisabolol synthase

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The expected taxonomic range for this enzyme is: Phyla dulcis

EC NUMBER
COMMENTARY
4.2.3.138
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RECOMMENDED NAME
GeneOntology No.
(+)-epi-alpha-bisabolol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-epi-alpha-bisabolol-forming)
Isolated from the plant Phyla dulcis (Aztec sweet herb). (+)-epi-alpha-Bisabolol is the precursor of the sweetener hernandulcin.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(+)-epi-alpha-bisabolol synthase
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LdTPS8
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
show the reaction diagram
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-
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?
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0048
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(2E,6E)-farnesyl diphosphate
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pH 7.4, 30°C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.04
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(2E,6E)-farnesyl diphosphate
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pH 7.4, 30°C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
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assay at
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
using Ni-NTA chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in yeast and Escherichia coli. The engineered yeast can synthesize a significant amount (0.3 mg per ml) of alpha-bisabolol in shake-flask cultivation
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