Information on EC 4.2.3.138 - (+)-epi-alpha-bisabolol synthase

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The expected taxonomic range for this enzyme is: Phyla dulcis

EC NUMBER
COMMENTARY hide
4.2.3.138
-
RECOMMENDED NAME
GeneOntology No.
(+)-epi-alpha-bisabolol synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = (+)-epi-alpha-bisabolol + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-epi-alpha-bisabolol-forming)
Isolated from the plant Phyla dulcis (Aztec sweet herb). (+)-epi-alpha-Bisabolol is the precursor of the sweetener hernandulcin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
(+)-epi-alpha-bisabolol + diphosphate
show the reaction diagram
-
single terpene product in vitro
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0048
(2E,6E)-farnesyl diphosphate
pH 7.4, 30C; pH 7.5, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
(2E,6E)-farnesyl diphosphate
Phyla dulcis
J7LH11
pH 7.4, 30C; pH 7.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in yeast and Escherichia coli. The engineered yeast can synthesize a significant amount (0.3 mg per ml) of alpha-bisabolol in shake-flask cultivation; expression in Saccharomyces cerevisiae and Escherichia coli