Information on EC 4.2.3.133 - alpha-copaene synthase

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The expected taxonomic range for this enzyme is: Gunneridae

EC NUMBER
COMMENTARY
4.2.3.133
-
RECOMMENDED NAME
GeneOntology No.
alpha-copaene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = (-)-alpha-copaene + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, alpha-copaene-forming)
Isolated from Helianthus annuus (sunflower). The enzyme also produces beta-caryophyllene, delta-cadinene and traces of other sesquiterpenoids. See EC 4.2.3.13 (+)-delta-cadinene synthase, EC 4.2.3.57 (-)-beta-caryophyllene synthase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alpha-copaene synthase
-
-
sesquiterpene synthase
Q4U3F6
-
sesquiterpene synthase 1
B9S9Z3
-
SeTPS1
B9S9Z3
-
TPS2
Q4U3F6
isoform
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
isoform TPS2; cultivar HA300
UniProt
Manually annotated by BRENDA team
cultivar Desiree
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(+)-delta-cadinene + diphosphate
show the reaction diagram
B9S9Z3
-
yield of 40% (+)-delta-cadinene
-
?
(2E,6E)-farnesyl diphosphate
(-)-alpha-copaene + diphosphate
show the reaction diagram
-
-
-
-
?
(2E,6E)-farnesyl diphosphate
(-)-alpha-copaene + diphosphate
show the reaction diagram
B9S9Z3
-
yield of 60% (-)-alpha-copaene
-
?
(2E,6E)-farnesyl diphosphate
alpha-copaene + diphosphate
show the reaction diagram
Q4U3F6
-
delta-cadinene is one of the two main products, while alpha-muuorlene, beta-caryophyllene, alpha-humulene and alpha-copaene are minor products
-
?
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q4U3F6
x * 64200, calculated from amino acid sequence
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA affinity column chromatography
Q4U3F6
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Saccharomyces cerevisiae strain EPY300 as a thioredoxin-fusion protein
Q4U3F6
expressed in Saccharomyces cerevisiae strain EPY300
B9S9Z3