Information on EC 4.2.3.116 - (+)-camphene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.3.116
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RECOMMENDED NAME
GeneOntology No.
(+)-camphene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (+)-camphene + diphosphate
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Metabolic pathways
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monoterpene biosynthesis
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Monoterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [cyclizing, (+)-camphene-forming]
Cyclase I of Salvia officinalis (sage) gives about equal parts (+)-camphene and (+)-alpha-pinene. (3R)-Linalyl diphosphate can also be used by the enzyme in preference to (3S)-linalyl diphosphate. Requires Mg2+ (preferred to Mn2+). See also EC 4.2.3.121 (+)-alpha-pinene synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
250375-52-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3R)-linalyl diphosphate
(+)-camphene + diphosphate
show the reaction diagram
-
(3R)-enantiomer is preferred over (3S)-enantiomer
products are (+)-camphene and (+)alpha-pinene
-
?
(3S)-linalyl diphosphate
(-)-camphene + diphosphate
show the reaction diagram
-
(3R)-enantiomer is preferred over (3S)-enantiomer
products are (-)-camphene and (-)alpha-pinene
-
?
geranyl diphosphate
(+)-camphene + diphosphate
show the reaction diagram
neryl diphosphate
(+)-camphene + diphosphate
show the reaction diagram
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-
products are (+)-camphene and (+)alpha-pinene
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-hydroxymercuribenzoate
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diphosphate
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N-ethylmaleimide
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
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assay in hexane
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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55% of maximum activity
58
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20% of maximum activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
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or below, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immature leaf
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95000
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gel filtration
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diisopropyl ether
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enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene
hexadecane
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enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene
hexane
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enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene. Reactions run in hexane generate product at roughly one-third the rate of companion assays in which the lyophilized protein is rehydrated in distilled water
additional information
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enzyme is active in hexane, hexadecane, diisopropyl ether, or distilled water, and gives rise to the same olefin distribution of 25% (+)-alpha-pinene, 25% (+)-camphene 43% (+)-limonene, 7% myrcene
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
lyophilization and rehydration in destilled water results in loss of about 10% of activity
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