Information on EC 4.2.3.106 - (E)-beta-ocimene synthase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
4.2.3.106
-
RECOMMENDED NAME
GeneOntology No.
(E)-beta-ocimene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
geranyl diphosphate = (E)-beta-ocimene + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
monoterpene biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [(E)-beta-ocimene-forming]
Widely distributed in plants, which release beta-ocimene when attacked by herbivorous insects.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
ama0a23
-
-
-
-
ama0a23
Q84NC8
-
AtTPS03
-
-
-
-
beta-ocimene synthase
-
-
-
-
EbetaOS
Q672F7
-
EBOS
Q5UB07
-
LjEbetaOS
-
-
-
-
MtEBOS
-
-
-
-
MTSL4
Q5CD81
-
MTSL4
Citrus unshiu Marc
Q5CD81
-
-
myrcene/(E)-beta-ocimene synthase
Q9ZUH4
-
TPS10
Q9ZUH4
-
TPS3
G0Y7D1
-
TPS4
Q5UB07
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
memeber of TPSg group of terpene synthases
UniProt
Manually annotated by BRENDA team
member of TPSb group of angiosperm monoterpene and hemiterpene synthases
A4FVP2
UniProt
Manually annotated by BRENDA team
member oft the TPSb subfamily of angiosperm monoterpene synthases
SwissProt
Manually annotated by BRENDA team
Citrus unshiu Marc
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-, Q672F7
enzyme is involved in the herbivore-induced indirect defense response of spider mite-infested Lotus japonicus via de novo formation and emission (E)-beta-ocimene
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranly diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
-, Q672F7
-
98% (E)-beta-ocimene plus 2% (Z)-beta-ocimene
-
?
geranly diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
Q84NC8
product formation does not proceed via an RR-dependent isomerization of geranyl diphosphate to 3S-linalyl diphosphate
97% (E)-beta-ocimene plus minor amounts of (Z)-beta-ocimene and myrcene
-
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
A4FVP2, -
-
94% (E)-beta-ocimene plus 4% (Z)-beta-ocimene and 2% myrcene
-
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
-, Q5UB07
-
97.5% of (E)-beta-ocimene plus 2% (Z)-beta-ocimene and 0.5% myrcene
-
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
Q9ZUH4
-
main products are 56% beta-myrcene and 20% (E)-beta-ocimene plus minor amounts of (-)-limonene, (+)-limnonene, 2-carene and tricyclene
-
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
G0Y7D1
-
products are alpha-thujene and (+)-sabinene
-
?
geranyyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
Citrus unshiu, Citrus unshiu Marc
Q5CD81
-
97.2% (E)-beta-ocimene
-
?
additional information
?
-
Q5CD81
no substrate: farnesyl diphosphate and geranylgeranyl diphosphate
-
-
-
additional information
?
-
-, Q672F7
no substrates: farnesyl diphosphate or geranylgeranyl diphosphate.
-
-
-
additional information
?
-
Citrus unshiu Marc
Q5CD81
no substrate: farnesyl diphosphate and geranylgeranyl diphosphate
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
Q5CD81
presence of Mn2+ and Mg2+ required
Mn2+
Q5CD81
presence of Mn2+ and Mg2+ required
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
-
Q5CD81
calculated
6.3
-
-, Q672F7
calculated
6.7
-
A4FVP2, -
calculated
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Q5CD81
abundant in flower, amount decreases towards fruit development
Manually annotated by BRENDA team
Citrus unshiu Marc
-
abundant in flower, amount decreases towards fruit development
-
Manually annotated by BRENDA team
Q5CD81
abundant in flower, amount decreases towards fruit development
Manually annotated by BRENDA team
G0Y7D1
developing fruits and mature fruits
Manually annotated by BRENDA team
Citrus unshiu Marc
-
abundant in flower, amount decreases towards fruit development
-
Manually annotated by BRENDA team
-, Q5UB07
(E)-b-ocimene is released from leaves of both undamaged and insect-damaged plants, but at levels two-fold higher in insect-damaged M.edicago truncatula
Manually annotated by BRENDA team
G0Y7D1
male leaves and female leaves
Manually annotated by BRENDA team
Citrus unshiu Marc
-
-
-
Manually annotated by BRENDA team
additional information
-, Q5UB07
transcripts of MtEBOS are not detected via RNA blots in stems, flowers, and roots
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-, Q672F7
x * 68800, calculated
?
Q5CD81
x * 71200, calculated, x * 71000, SDS-PAGE
?
A4FVP2, -
x * 63020, calculated
?
G0Y7D1
x * 62000, calculated, x * 63000, SDS-PAGE of recombinant protein
?
Citrus unshiu Marc
-
x * 71200, calculated, x * 71000, SDS-PAGE
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
-, Q672F7
sequence contains a plastid transit peptide
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli
Q5CD81
expression in Escherichia coli
-, Q672F7
expression in Escherichia coli
-, Q5UB07
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
only slight variations in monoterpene synthase mRNA levels during the daily light/dark cycle
Q84NC8
transcripts are upregulated in response to mechanical wounding and treatment with jasmonic acid, concurrent with induced emission of (E)-beta-ocimene
A4FVP2, -
transcripts of LjEbOS are induced in plants infested with two-spotted spider mites, Tetranychus urticae, coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin, a potent fungal elicitor of plant volatile emission, also induces transiently increased levels of transcripts, but not elevated release of (E)-beta-ocimene
-, Q672F7
treatment with the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid does not cause an increase in transcripts
-, Q5UB07
transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
-, Q5UB07