Information on EC 4.2.3.103 - ent-isokaurene synthase

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The expected taxonomic range for this enzyme is: Oryza sativa Indica Group

EC NUMBER
COMMENTARY
4.2.3.103
-
RECOMMENDED NAME
GeneOntology No.
ent-isokaurene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ent-copalyl diphosphate = ent-isokaurene + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
Diterpenoid biosynthesis
-
kauralexin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate diphosphate-lyase (cyclizing, ent-isokaurene-forming)
Two enzymes of the rice sub-species Oryza sativa ssp. indica, OsKSL5 and OsKSL6, produce ent-isokaurene. A variant of OsKSL5 from the sub-species Oryza sativa ssp. japonica produces ent-pimara-8(14),15-diene instead [cf. EC 4.2.3.30, ent-pimara-8(14),15-diene synthase].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
kaurene synthase-like
A4KAG7, A4KAG8
gene name
KSL5
A4KAG7
isoform
KSL6
A4KAG8
isoform
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
isofom KSL5; cultivar IR24
UniProt
Manually annotated by BRENDA team
isofom KSL5i
UniProt
Manually annotated by BRENDA team
isofom KSL6; cultivar IR24
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
ent-isokaurene + diphosphate
show the reaction diagram
A4KAG7, A4KAG8
-
-
-
?
ent-copalyl diphosphate
ent-isokaurene + diphosphate
show the reaction diagram
A4KAG7, A4KAG8
-
-
-
?
ent-copalyl diphosphate
ent-isokaurene + diphosphate
show the reaction diagram
A4KAG7, A4KAG8
more than 90% yield
-
-
?
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
GST-agarose bead chromatography; GST-agarose bead chromatography
A4KAG7, A4KAG8
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli C41 cells; expressed in Escherichia coli C41 cells
A4KAG7, A4KAG8
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
I664T
A4KAG7, A4KAG8
the mutant specifically produces pimara-8(14),15-diene from ent-copalyl diphosphate; the mutant specifically produces pimara-8(14),15-diene from ent-copalyl diphosphate
I718V
A4KAG7, A4KAG8
the mutant specifically produces ent-isokaur-15-ene from ent-copalyl diphosphate
V661L
A4KAG7, A4KAG8
the mutant specifically produces ent-isokaur-15-ene from ent-copalyl diphosphate