Information on EC 4.2.3.102 - sesquithujene synthase

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The expected taxonomic range for this enzyme is: Zea mays

EC NUMBER
COMMENTARY hide
4.2.3.102
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RECOMMENDED NAME
GeneOntology No.
sesquithujene synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesyl diphosphate = sesquithujene + diphosphate
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (sesquithujene-forming)
The enzyme from Zea mays, variety Delprim, gives mainly sesquithujene with (S)-beta-bisabolene and (E)-beta-farnesene plus traces of other sesquiterpenoids, cf. EC 4.2.3.55 [(S)-beta-bisabolene synthase] and EC 4.2.3.47 (beta-farnesene synthase). It requires Mg2+ or Mn2+. The exact product ratio is dependent on which metal ion is present.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
variety Delprim
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
sesquithujene + diphosphate
show the reaction diagram
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-
sesquithujene is the major product, the enzyme also produces (S)-beta-bisabolene, and the minor products 7-epi-sesquithujene, (Z)-alpha-bergamotene, (E)-alpha-bergamotene, sesquisabinene B, sesquisabinene A, and (E)-beta-farnesene
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?
geranyl diphosphate
(S)-(-)-limonene + diphosphate
show the reaction diagram
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main product is (S)-(-)-limonene, minor products are alpha-thujene, beta-myrcene, (+)-sabinene, (-)-sabinene, alpha-terpinene, (E)-beta-ocimene, beta-phellandrene, alpha-terpinolene, gamma-terpinene, sabinene hydrate, (R)-(-)-linalool, and (S)-(1)-linalool
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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either Mg2+ or Mn2+ is required for activity, the optimum is 5 mM Mg2+
Mn2+
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either Mg2+ or Mn2+ is required for activity, the optimum is 0.25 mM Mg2+
additional information
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no enzyme activity in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest expression
Manually annotated by BRENDA team
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ear leaf
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Econopac 10DG column chromatography and Strep-Tactine affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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