Information on EC 4.2.2.B6 - muropeptide lyase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.2.B6
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
muropeptide lyase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GlcNAc-beta-(1->4)-Mur2Acoyl-L-Ala-gamma-D-Glu = N-acetyl-D-glucosamine + 1,6-anhydro-N-acetylmaramoyl-L-Ala-gamma-D-Glu
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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lytic transglycosylases, MltE and MltC, in combination increase transcriptional regulator CsgD expression and distinct colony morphotype on Congo red agar plates, named rdar. MltE and MltC work redundantly to regulate transcriptional regulator CsgD at the transcriptional and posttranscriptional levels. The effect of MltE and MltC on CsgD levels is independent of the known regulatory pathways that sense cell envelope stress. The mltE deletion mutant does not show a change in rdar morphotype expression. The overexpression of MltE from a plasmid leads to an upregulated rdar morphotype after 17 h, and upon extended incubation it manifests as an extended matrix structure on Congo red agar plates
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cell wall peptidoglycan
?
show the reaction diagram
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MltE can cleave in the middle of the polymeric substrate
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?
Micrococcus luteus peptidoglycan
?
show the reaction diagram
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?
additional information
?
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
a periplasmic, outer membrane-attached enzyme
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
in solution, the enzyme behaves as a monomer, as judged from size exclusion chromatography and dynamic light scattering
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with the glycopeptide inhibitor bulgecin A and N-acetylglucosaminyl-N-acetylmuramyl-L-Ala-D-Glu, hanging drop vapor diffusion method, using 0.1 M lithium sulfate, 15% (w/v) PEG 8000 in 20 mM HEPES buffer, pH 7.5
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mutant enzyme E64Q is expressed in Escherichi coli strain B834(DE3) pLysS
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE