Information on EC 4.2.2.14 - glucuronan lyase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
4.2.2.14
-
RECOMMENDED NAME
GeneOntology No.
glucuronan lyase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Eliminative cleavage of (1->4)-beta-D-glucuronans to give oligosaccharides with 4-deoxy-beta-D-gluc-4-enuronosyl groups at their non-reducing ends. Complete degradation of glucuronans results in the formation of tetrasaccharides.
show the reaction diagram
complete degradation of glucuronans results in the formation of tetrasaccharides
-
Eliminative cleavage of (1->4)-beta-D-glucuronans to give oligosaccharides with 4-deoxy-beta-D-gluc-4-enuronosyl groups at their non-reducing ends. Complete degradation of glucuronans results in the formation of tetrasaccharides.
show the reaction diagram
-
-
-
-
Eliminative cleavage of (1->4)-beta-D-glucuronans to give oligosaccharides with 4-deoxy-beta-D-gluc-4-enuronosyl groups at their non-reducing ends. Complete degradation of glucuronans results in the formation of tetrasaccharides.
show the reaction diagram
complete degradation of glucuronans results in the formation of tetrasaccharides
Sinorhizobium meliloti M5N1CS
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
elimination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-beta-D-glucuronan lyase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
(1,4)-beta-D-glucuronan lyase
-
-
-
-
(1,4)-beta-D-glucuronan lyase
-
-
(1,4)-beta-D-glucuronan lyase
Sinorhizobium meliloti M5N1CS
-
-
-
(1,4)-beta-D-glucuronan lyase
-
-
(1,4)-beta-D-glucuronan lyase
Trichoderma sp. GL2
-
-
-
(1->4)-beta-D-glucuronan lyase
-
-
-
-
endo-beta-(1->4)-glucuronan lyase
-
endo-beta-1,4-glucuronan lyase
-
endopolyglucuronate lyase
-
-
endopolyglucuronate lyase
Sinorhizobium meliloti M5N1CS
-
-
-
GL2 glucuronan lyase
-
-
glucuronan lyase
-
glucuronan lyase
-
-
CAS REGISTRY NUMBER
COMMENTARY
193766-71-1
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
M5N1CS (NCIMB 40472) mutant strain
-
-
Manually annotated by BRENDA team
strain M5N1CS, NCIMB 40472
-
-
Manually annotated by BRENDA team
Sinorhizobium meliloti M5N1CS
M5N1CS (NCIMB 40472) mutant strain
-
-
Manually annotated by BRENDA team
Sinorhizobium meliloti M5N1CS
strain M5N1CS, NCIMB 40472
-
-
Manually annotated by BRENDA team
Trichoderma sp. GL2
-
-
-
Manually annotated by BRENDA team
Trichoderma sp. GL2
GL2
-
-
Manually annotated by BRENDA team
Trichoderma sp. GL2
strain GL2
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-O-acetylated glucuronan
?
show the reaction diagram
Sinorhizobium meliloti, Sinorhizobium meliloti M5N1CS
-
beta(1-4)-linked
-
-
?
acetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
-
-
-
?
acetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
Trichoderma sp., Trichoderma sp. GL2
-
-
-
?
acetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
Sinorhizobium meliloti M5N1CS
-
-
-
?
cellouronate
?
show the reaction diagram
substrate specificity of recombinant TrGL is examined using various polyuronates, including alginate, hyaluronate, pectin, polygalacturonic acid, amylouronate, and carboxymethyl cellulose. Results indicate that there is high substrate specificity of the enzyme for cellouronate
-
-
?
deacetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
-
-
-
?
deacetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
Trichoderma sp., Trichoderma sp. GL2
-
-
-
?
deacetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
Sinorhizobium meliloti M5N1CS
-
-
-
?
deacetylated polyglucuronate
4,5-unsaturated oligoglucuronates
show the reaction diagram
Sinorhizobium meliloti, Sinorhizobium meliloti M5N1CS
-
beta-(1-4)-linked, best substrate, enzyme shows specific endopolyglucuronate lyase activity
-
?
glucuronan
4-deoxy-beta-D-hex-4-enopyranosylglucuronate-(1-4)-O-beta-D-glucuropyranosyluronate-(1-4)-O-beta-D-glucuropyranosyluronate + ?
show the reaction diagram
Trichoderma sp., Trichoderma sp. GL2
-
-
-
?
glucuronan
oligoglucuronan
show the reaction diagram
-
-
-
?
polyglucuronic acid
?
show the reaction diagram
Trichoderma sp., Trichoderma sp. GL2
-
-
-
-
?
glucuronan
oligoglucuronan
show the reaction diagram
-
-
-
?
additional information
?
-
Sinorhizobium meliloti, Sinorhizobium meliloti M5N1CS
-
no activity with polyglucuronate methyl ester alpha-(1-4)-linked, hyaluronate beta-(1-3), beta(1-4)-linked, glucogluronan beta-(1-3), beta(1-4)-linked, alpha-L-guluronate blocks alpha-(1-4)-linked, beta-D-mannuronate blocks beta(1-4)-linked, 2,3-di-O-acetylated glucuronan beta(1-4)-linked, glucuronan beta(1-4)-linked
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetylated (1->4)-beta-D-glucuronan
4-deoxy-beta-D-gluc-4-enuronosyl (1->4)-beta-D-glucuronan
show the reaction diagram
Sinorhizobium meliloti, Sinorhizobium meliloti M5N1CS
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
1 mM, enhances activity 4.56fold
Ca2+
-
activity and thermostability increases in the presence of Ca2+
Ca2+
-
a calcium ion contributes to the enzyme stability
Co2+
-
1 mM, enhances activity 1.5fold
Li+
-
1 mM, enhances activity 4.4fold
Mg2+
-
1 mM, enhances activity 2.5fold
Mn2+
-
1 mM, enhances activity 2.2fold
additional information
-
enzyme appears not to be really affected by ionic strength variations. The optimum is 300 mM concentration of potassium acetate buffer
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ag+
-
100% reduced activity at 1 mM
Ca2+
-
21-30% reduced activity at 1 mM
Cd2+
-
26-29% reduced activity at 1 mM
Cu2+
-
85% reduced activity at 1 mM
Cu2+
-
1 mM, 81% inhibition
EDTA
-
21-30% reduced activity at 1 mM
Fe2+
-
1 mM, 19% inhibition
Hg2+
-
94% reduced activity at 1 mM
Li+
-
26-29% reduced activity at 1 mM
NaN3
-
26-29% reduced activity at 1 mM
Ni2+
-
1 mM, 60% inhibition
Rb2+
-
1 mM, 10% inhibition
-
Zn2+
-
59% reduced activity at 1 mM
Mn2+
-
21-30% reduced activity at 1 mM
additional information
-
no inhibition by Mg2+
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
immobilized glucuronan lyase activity is calculated for deacetylated glucuronan. The activity is 1300times less than the theoretical immobilized activity. Activity decreases 4times and 14times for native and highly acetylated glucuronans, respectively
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
-
assay at
6.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.9
-
isoelectric focusing
6.95
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Trichoderma sp. GL2
-
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20000
-
gel filtration
649368
27000
-
SDS-PAGE
690555
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 27000, SDS-PAGE
?
Trichoderma sp. GL2
-
x * 27000, SDS-PAGE
-
monomer
-
1 * 20000, SDS-PAGE
monomer
Sinorhizobium meliloti M5N1CS
-
1 * 20000, SDS-PAGE
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 20% (w/v) PEG3350 and 0.2 M ammonium citrate buffer (pH 5.0)
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 8
-
30 min, stable
663738
4 - 8
-
-
703497
5 - 9
-
stable over a broad when treated at 4C for 24 h
690555
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10 - 40
-
enzyme activity remains after incubation at 10 to 40C for 10 min, but approximately 60% of the activity is lost at 50C
690555
35
-
1 h, stable below
663738
40
-
1 h, 90% loss of activity
663738
52
-
50% of maximal activity
649368
60
-
1 h, complete inactivation
663738
67
-
complete inactivation
649368
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after 4 h of immobilization on activated epoxy-Sepharose, no decrease of activity is measured in the buffer containing epoxy activated Sepharose, immobilizations on CNBr and NHS activated matrices show a rapid decrease of the glucuronan lyase activity in coupling buffers
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 50 mM KCl, purified enzyme, stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, 394fold
-
phenyl-Toyopearl column chromatography and SuperQ-Toyopearl column chromatography
-
gel filtration and anion-exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
-
a glucuronan lyase is immobilized on a monolithic Convective Interaction Media disk. Degradations of three glucuronans with various O-acetylation degrees is investigated and compared with degradations using free enzyme. The immobilized glucuronan lyase is inhibited by the O-acetylation degree like the free enzyme. 1H NMR analyses are used to study the O-acetylation degree of oligoglucuronans and demonstrate that the average degrees of polymerization are inclusive between 4 and 13 after 24 h of degradation. This first immobilization of a glucuronan lyase constitutes a tool to produce oligoglucuronans
synthesis
-
production of oligoglucuronans by enzymatic depolymerization of nascent glucuronan
synthesis
-
production of glucuronan oligosaccharides
synthesis
-
large-scale production of oligocellouronic acids
synthesis
-
production of highly O-acetylated oligoglucuronan in order to envisage new therapeutic applications fields such as the realization of biological tests among animals and vegetals
synthesis
Trichoderma sp. GL2
-
large-scale production of oligocellouronic acids; production of highly O-acetylated oligoglucuronan in order to envisage new therapeutic applications fields such as the realization of biological tests among animals and vegetals; production of oligoglucuronans by enzymatic depolymerization of nascent glucuronan
-