Information on EC 4.2.1.B26 - (R)-2-hydroxyglutaryl-CoA dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.B26
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
(R)-2-hydroxyglutaryl-CoA dehydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-hydroxyglutaryl-CoA = (E)-glutaconyl-CoA + H2O
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glutamate and glutamine metabolism
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SYSTEMATIC NAME
IUBMB Comments
(R)-2-hydroxyglutaryl-CoA hydro-lyase ((E)-glutaconyl-CoA-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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P11569 and P11570
UniProt
Manually annotated by BRENDA team
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P11569 and P11570
UniProt
Manually annotated by BRENDA team
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Q9X5B7 and Q9X5B8
UniProt
Manually annotated by BRENDA team
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Q9X5B7 and Q9X5B8
UniProt
Manually annotated by BRENDA team
-
Q9X5B7 and Q9X5B8
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E)-oxalocrotonyl-CoA + H2O
(2R)-2-hydroxy-5-oxoadipyl-CoA
show the reaction diagram
(E,E)-muconyl-CoA + H2O
(2R,4E)-2-hydroxy-4-hexenedioyl-CoA
show the reaction diagram
(R)-2-hydroxyadipyl-CoA
(E)-2-hexenedioyl-CoA + H2O
show the reaction diagram
(R)-2-hydroxyglutaryl-CoA
(E)-glutaconyl-CoA + H2O
show the reaction diagram
(R)-2-hydroxyglutaryl-N-acetylcysteamine
(E)-glutaconyl-N-acetylcysteamine + H2O
show the reaction diagram
butynedioyl-CoA
oxaloacetate + CoA
show the reaction diagram
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initial products are 2-hydroxyfumaroyl-CoA or 2-hydroxymalyl-CoA which tautomerize to oxaloacetyl-CoA. The latter spontaneously hydrolyzes to oxaloacetate and coenzyme A
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?
S-(R)-2-hydroxyglutarylpantetheine
S-(E)-glutaconylpantetheine + H2O
show the reaction diagram
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r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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2.2 mol per mol of heterodimeric enzyme
Fe-S cluster
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7.3 mol iron per mol of heterodimeric enzyme
Manganese
-
0.7 mol per mol of heterodimeric enzyme
Zinc
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1.2 mol per mol of heterodimeric enzyme
[4Fe-4S]2+ cluster
additional information
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molybdenum or copper not detected
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-2-hydroxyglutaryl-CoA dehydratase component A
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FMNH2
riboflavin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
(E)-glutaconyl-CoA
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
1.1
(E)-oxalocrotonyl-CoA
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
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0.57
(E,E)-muconyl-CoA
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
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0.1
(R)-2-hydroxyadipyl-CoA
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
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0.052
(R)-2-hydroxyglutaryl-CoA
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
0.115
(R)-2-hydroxyglutaryl-N-acetylcysteamine
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
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2.1
butynedioyl-CoA
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
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0.09
S-(R)-2-hydroxyglutarylpantetheine
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
82
(R)-2-hydroxyglutaryl-CoA
Clostridium symbiosum
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
27
(E)-glutaconyl-CoA
Clostridium symbiosum
-
strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
10074
0.08
(E)-oxalocrotonyl-CoA
Clostridium symbiosum
-
strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
206973
8.2
(E,E)-muconyl-CoA
Clostridium symbiosum
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
206972
440
(R)-2-hydroxyadipyl-CoA
Clostridium symbiosum
-
strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
206971
1600
(R)-2-hydroxyglutaryl-CoA
Clostridium symbiosum
-
strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
52055
560
(R)-2-hydroxyglutaryl-N-acetylcysteamine
Clostridium symbiosum
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
206969
0.14
butynedioyl-CoA
Clostridium symbiosum
-
strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
206974
800
S-(R)-2-hydroxyglutarylpantetheine
Clostridium symbiosum
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strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
206970
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32
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strictly anaerobic conditions, substrate (R)-2-hydroxyglutaryl-CoA, 200 mM potassium phosphate pH 7.0, 22°C
54
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substrate (R)-2-hydroxyglutaryl-CoA, strictly anaerobic conditions, 25°C, Tris-HCl buffer, pH 8.0, (R)-2-hydroxyglutaryl-CoA dehydratase activator protein from Acidaminococcus fermentans
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43033
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1 * 53981 (gene hgdA) + 1 * 43033 (gene hgdB), calculation from amino acid sequence
53981
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1 * 53981 (gene hgdA) + 1 * 43033 (gene hgdB), calculation from amino acid sequence
54000
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1 * 54000, 1 * 42000, SDS-PAGE
55000
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1 * 55000, 1 * 42000, SDS-PAGE
100000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen sensitive with a half-life of 2 days under aerobic conditions
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733766
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, liquid nitrogen, long-term storage
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4°C anaerobic conditions, 1 week stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
chromatography on blue-Sepharose
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partail purification
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expression of genes for the production of glutaconate from 2-oxoglutarate in Escherichia coli
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