Information on EC 4.2.1.B22 - galactarate dehydratase (2-oxo-D-threo-4,5-dihydroadipate)

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The expected taxonomic range for this enzyme is: Oceanobacillus iheyensis

EC NUMBER
COMMENTARY hide
4.2.1.B22
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
galactarate dehydratase (2-oxo-D-threo-4,5-dihydroadipate)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
galactarate = 2-oxo-D-threo-4,5-dihydroxyadipate + H2O
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
galactarate hydro-lyase (2-oxo-D-threo-4,5-dihydroadipate)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
galactarate
2-oxo-D-threo-4,5-dihydroxyadipate + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
galactarate
2-oxo-D-threo-4,5-dihydroxyadipate + H2O
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.62 - 4.4
galactarate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0013 - 6.8
galactarate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00029 - 11
galactarate
3374
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion at room temperature: (1) SeMet-substituted wild-type enzyme in a complex with Mg2+, (2) wild-type enzyme (containing Met) in a complex with Mg2+, (3) a tetragonal form of the wild type in a complex with Mg2+ and L-malate, (4) a triclinic form of enzyme/Mg2+/L-malate, and (5) the Y90F mutant in a complex with Met with Mg2+ and galactarate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H45Q
kcat/Km is 38000fold lower than wild-type value
R162N
kcat/Km is 17000fold lower than wild-type value
Y164F
no detectable activity
Y90F
kcat/Km is 3500fold lower than wild-type value
Y164F
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no detectable activity
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