Information on EC 4.2.1.98 - 16alpha-hydroxyprogesterone dehydratase

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The expected taxonomic range for this enzyme is: Eubacterium

EC NUMBER
COMMENTARY hide
4.2.1.98
-
RECOMMENDED NAME
GeneOntology No.
16alpha-hydroxyprogesterone dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
16alpha-Hydroxyprogesterone = 16,17-didehydroprogesterone + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-O bond cleavage by elimination of water
SYSTEMATIC NAME
IUBMB Comments
16alpha-hydroxyprogesterone hydro-lyase (16,17-didehydroprogesterone-forming)
16alpha-Hydroxypregnenolone is also a substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
89287-36-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
16-dehydroprogesterone + H2O
16alpha-hydroxyprogesterone
show the reaction diagram
16alpha-Hydroxypregnanolone
Iso-pregnanolone
show the reaction diagram
16alpha-hydroxypregnenolone
16-dehydropregnenolone + H2O
show the reaction diagram
16alpha-Hydroxypregnenolone
?
show the reaction diagram
16alpha-hydroxypregnenolone
solasodin + H2O
show the reaction diagram
16alpha-hydroxyprogesterone
16,17-didehydroprogesterone + H2O
show the reaction diagram
16alpha-Hydroxyprogesterone
16-Dehydroprogesterone
show the reaction diagram
16alpha-hydroxyprogesterone
16dehydroprogesterone + H2O
show the reaction diagram
16alpha-hydroxyprogesterone
?
show the reaction diagram
16alpha-hydroxyprogesterone
DELTA16-progesterone + H2O
show the reaction diagram
-
in the hydration direction the enzyme is only 15% as active as in the dehdyration direction
-
-
r
DELTA16-Progesterone
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
16alpha-hydroxyprogesterone
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16-Ketoprogesterone
-
; competitive with respect to 16alpha-hydroxypregnenolone and 16alpha-hydroxyprogesterone
16alpha-hydroxypregnenolone
-
substrate inhibition at high concentrations
butanol
-
-
Cetyltrimethylammonium bromide
-
; no inhibition by NaBr
HgCl2
-
; 0.1 mM, 87% inhibition
iodoacetic acid
-
slight
Isopropanol
-
-
n-butanol
-
-
n-Propanol
-
-
NEM
-
slight
PCMB
-
0.5 mM, 50% inhibition
Propanol
-
-
rifampicin
-
-
Rifampin
-
i.e. rifampicin
Sulfhydryl reagent
-
-
-
sulfhydryl reagents
-
-
Tris-HCl
additional information
-
not inhibited by inhibitors of electron transport reactions or metal chelators
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methanol
-
active at 15-30% v/v methanol with an optimal concentration of 25%
SDS
-
0.05%, about 7fold stimulation; 0.1%, stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
16alpha-hydroxypregnenolone
-
-
0.52 - 0.56
16alpha-hydroxyprogesterone
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 0.18
16-Ketoprogesterone
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.7094
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
-
activity is unaffected by pH in potassium phosphate buffer over the range pH 5.0-8.5
5.8 - 8
-
the enzyme exhibits a broad pH range without a distinct pH-optimum
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
-
enzyme activity is not affected over this range in potassium phosphate buffer, but is inhibited by Tris-HCl buffer at pH above 7.0
5.8 - 8
-
broad pH-range without a distinct pH-optimum; the enzyme exhibits a broad pH range without a distinct pH-optimum. Above pH 8.5 the activity decreases and is undetectable at pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 45
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 50
-
35°C: about 65% of maximal activity, 50°C: about 40% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42400
-
4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE; 8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE; x * 42400, SDS-PAGE
181000
-
active in two molecular weight forms, gel filtration; tetrameric form, gel filtration
285000
-
gel filtration
326000
-
active in two molecular weight forms, gel filtration; octameric form, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
10 min, stable
65
-
10 min, 70% inactivation; 6 min, 70% loss of activity
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Methanol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE