Information on EC 4.2.1.95 - kievitone hydratase

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The expected taxonomic range for this enzyme is: Fusarium phaseoli

EC NUMBER
COMMENTARY
4.2.1.95
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RECOMMENDED NAME
GeneOntology No.
kievitone hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
kievitone hydrate = kievitone + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C-O bond cleavage by elimination of water
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SYSTEMATIC NAME
IUBMB Comments
kievitone-hydrate hydro-lyase (kievitone-forming)
The enzyme from Fusarium sp. hydrates the methylbutenyl sidechain of the isoflavonoid phytoalexins, thus reducing their toxicity.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydratase, kievitone
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KHase
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CAS REGISTRY NUMBER
COMMENTARY
70431-10-6
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
kievitone + H2O
kievitone hydrate
show the reaction diagram
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?
kievitone + H2O
kievitone hydrate
show the reaction diagram
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-
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?
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
kievitone + H2O
kievitone hydrate
show the reaction diagram
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-
-
?
additional information
?
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the enzyme is a virulence factor, enzyme catalyses detoxification of a bean phytoalexin
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme is a virulence factor, enzyme catalyses detoxification of a bean phytoalexin
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-
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0175
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Kievitone
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pH 5.5, 27°C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
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at 27°C
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
27
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assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.1
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
97000
102000
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non-denaturing PAGE
97000
102000
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97000
102000
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electrophoresis
173000
175000
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173000
175000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
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2 * 47000-49000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Neurospora crassa and Emericella nidulans
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