Information on EC 4.2.1.95 - kievitone hydratase

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The expected taxonomic range for this enzyme is: Fusarium phaseoli

EC NUMBER
COMMENTARY
4.2.1.95
-
RECOMMENDED NAME
GeneOntology No.
kievitone hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
kievitone hydrate = kievitone + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C-O bond cleavage by elimination of water
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
kievitone-hydrate hydro-lyase (kievitone-forming)
The enzyme from Fusarium sp. hydrates the methylbutenyl sidechain of the isoflavonoid phytoalexins, thus reducing their toxicity.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydratase, kievitone
-
-
-
-
KHase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
70431-10-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
kievitone + H2O
kievitone hydrate
show the reaction diagram
-
-
-
?
additional information
?
-
-
the enzyme is a virulence factor, enzyme catalyses detoxification of a bean phytoalexin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme is a virulence factor, enzyme catalyses detoxification of a bean phytoalexin
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0175
Kievitone
-
pH 5.5, 27°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5
-
at 27°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
27
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.1
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
97000 - 102000
-
non-denaturing PAGE
5941
97000 - 102000
-
-
648516
97000 - 102000
-
electrophoresis
648517
173000 - 175000
-
-
648517
173000 - 175000
-
gel filtration
648518
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
2 * 47000-49000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Neurospora crassa and Emericella nidulans
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