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Information on EC 4.2.1.91 - arogenate dehydratase and Organism(s) Pseudomonas aeruginosa and UniProt Accession Q01269

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.91 arogenate dehydratase
IUBMB Comments
Also acts on prephenate and D-prephenyllactate. cf. EC 4.2.1.51, prephenate dehydratase.
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This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: Q01269
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
Synonyms
arogenate dehydratase, cyclohexadienyl dehydratase, atadt1, atadt2, atadt6, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
arogenate dehydratase
-
cyclohexadienyl dehydratase
-
prephenate dehydratase
-
arogenate dehydratase
-
-
-
-
carboxycyclohexadienyl dehydratase
-
-
-
-
cyclohexadienyl dehydratase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-O bond cleavage by elimination of water
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-arogenate hydro-lyase (decarboxylating; L-phenylalanine-forming)
Also acts on prephenate and D-prephenyllactate. cf. EC 4.2.1.51, prephenate dehydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
76600-70-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-arogenate
L-phenylalanine + H2O + CO2
show the reaction diagram
prephenate
phenylpyruvate + H2O + CO2
show the reaction diagram
pH 7.5, 37°C
-
-
?
L-arogenate
L-phenylalanine + CO2 + H2O
show the reaction diagram
prephenate
phenylpyruvate + CO2 + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arogenate
L-phenylalanine + H2O + CO2
show the reaction diagram
L-arogenate
L-phenylalanine + CO2 + H2O
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-arogenate
reaction with prephenate is inhibited
prephenate
reaction with L-arogenate is inhibited
L-arogenate
prephenate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19 - 0.22
L-arogenate
0.4 - 0.42
prephenate
0.22
L-arogenate
0.42
prephenate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
L-arogenate
0.4
prephenate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102.8
purified recombinant enzyme, with L-arogenate as substrate
307.7
purified recombinant enzyme, with prephenate as substrate
0.0483
L-arogenate dehydratase activity
0.158
prephenate dehydratase activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
x * 28000, SDS-PAGE
29500
2 * 29500, SDS-PAGE, gel filtration
30480
2 * 30480, calculated from amino acis sequence
72000
29500
30480
calculated from cloned gene pheC
72000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 28000, SDS-PAGE
dimer
dimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to electrophoretic homogeneity
native and recombinant enzyme
recombinant protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
pheC cloned and expressed by functional complementation of a pheA auxotroph of Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zamir, L.O.; Tiberio, R.; Fiske, M.; Berry, A.; Jensen, R.A.
Enzymatic and nonenzymatic dehydration reactions of L-arogenate
Biochemistry
24
1607-1612
1985
Brevundimonas diminuta, Pseudomonas aeruginosa
Manually annotated by BRENDA team
Fischer, R.; Jensen, R.
Arogenate dehydratase
Methods Enzymol.
142
495-502
1987
Brevundimonas diminuta, Euglena gracilis, Pseudomonas aeruginosa, Xanthomonas campestris
Manually annotated by BRENDA team
Zhao, G.; Xia, T.; Fischer, R.S.; Jensen, R.A.
Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular cloning of the gene and characterization of the gene product
J. Biol. Chem.
267
2487-2493
1992
Pseudomonas aeruginosa, Pseudomonas aeruginosa (Q01269)
Manually annotated by BRENDA team
Zhao, G.; Xia, T.; Aldrich, H.; Jensen, R.A.
Cyclohexadienyl dehydratase from Pseudomonas aeruginosa is a periplasmic protein
J. Gen. Microbiol.
139
807-813
1993
Pseudomonas aeruginosa (Q01269), Pseudomonas aeruginosa
Manually annotated by BRENDA team
Obmolova, G.; Teplyakov, A.; Xia, T.; Jensen, R.
Preliminary crystallographic study of cyclohexadienyl dehydratase from Pseudomonas aeruginosa
J. Mol. Biol.
232
992-994
1993
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Tam, R.; Saier, M.H., Jr.
A bacterial periplasmic receptor homologue with catalytic activity: cyclohexadienyl dehydratase of Pseudomonas aeruginosa is homologous to receptors specific for polar amino acids
Res. Microbiol.
144
165-169
1993
Pseudomonas aeruginosa (Q01269), Pseudomonas aeruginosa
Manually annotated by BRENDA team