Reference on EC 4.2.1.9 - dihydroxy-acid dehydratase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Myers, J.W.
Dihydroxy acid dehydrase: an enzyme involved in the biosynthesis of isoleucine and valine
J. Biol. Chem.
236
1414-1418
1961
Escherichia coli
Kanamori, M.; Wixom, R.L.
Studies in valine biosynthesis. V. Characteristics of the purified dihydroxyacid dehydratase from spinach leaves
J. Biol. Chem.
238
998-1005
1963
Spinacia oleracea
Altmiller, D.H.; Wagner, R.P.
Purification and properties of dihydroxy acid dehydratase from soluble and mitochondrial fractions of Neurospora crassa
Arch. Biochem. Biophys.
138
160-170
1970
Neurospora crassa
Wixom, R.L.; Garrett, J.L.; Fetzek, J.P.
A rapid determination of dihydroxyacid dehydratase activity in microbial cell suspensions
Anal. Biochem.
42
262-274
1971
Klebsiella aerogenes, Escherichia coli
Kiritani, K.; Wagner, R.P.
alpha,beta-Dihydroxy acid dehydratase (Neurospora crassa and spinach)
Methods Enzymol.
17A
755-764
1970
Neurospora crassa, Spinacia oleracea
-
Wixom, R.L.; Heinemann, M.A.; Semeraro, R.J.; Joseph, A.A.
Studies in valine biosynthesis. IX. The enzymes in photosynthetic and autotrophic bacteria
Biochim. Biophys. Acta
244
532-546
1971
Cupriavidus necator, Achromobacter ruhlandii, Chromatium sp., Acidovorax facilis, Paracoccus denitrificans, Cereibacter sphaeroides, Rhodospirillum rubrum, Chromatium sp. D
Cassady, W.E.; Leiter, E.H.; Bergquist, A.; Wagner, R.P.
Separation of mitochondrial membranes of Neurospora crassa. II. Submitochondrial localization of the isoleucine-valine biosynthetic pathway
J. Cell Biol.
53
66-72
1972
Neurospora crassa
Twarog, R.
Enzymes of the isoleucine-valine pathway in Acinetobacter
J. Bacteriol.
111
37-46
1972
Acinetobacter sp., Acinetobacter sp. 15150
Altmiller, D.H.
Neurospora mutants with mitochondria deficient in dihydroxy acid dehydratase. Properties of dihydroxy acid dehydratase from mutant strain 332
Biochem. Biophys. Res. Commun.
49
1000-1006
1972
BRENDA: Neurospora crassa
Textmining: Neurospora, Delftia lacustris
Armstrong, F.B.
Stereochemistry of the reductoisomerase and alpha,beta-dihydroxyacid dehydratase-catalysed steps in valine and isoleucine biosynthesis. Observation of a novel tertiary ketol rearrangement
J. Chem. Soc. Chem. Commun.
1974
351-352
1974
Salmonella enterica subsp. enterica serovar Typhimurium
-
Ryan, E.D.; Kohlhaw, G.B.
Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast
J. Bacteriol.
120
631-637
1974
Saccharomyces cerevisiae
Armstrong, F.B.; Muller, U.S.; Reary, J.B.; Whitehouse, D.; Crout, D.H.G.
Stereoselectivity and stereospecificity of the alpha,beta-dihydroxyacid dehydratase from Salmonella typhimurium
Biochim. Biophys. Acta
498
282-293
1977
Salmonella enterica subsp. enterica serovar Typhimurium
Brown, O.R.; Yein, F.
Dihydroxyacid dehydratase: the site of hyperbaric oxygen poisoning in branch-chain amino acid biosynthesis
Biochem. Biophys. Res. Commun.
85
1219-1224
1978
Escherichia coli
Flint, D.H.; Emptage, M.H.
Dihydroxy acid dehydratase from spinach contains a [2Fe-2S] cluster
J. Biol. Chem.
263
3558-3564
1988
Spinacia oleracea
Kuo, C.F.; Mashino, T.; Fridovich, I.
An activity stain for dihydroxy-acid dehydratase
Anal. Biochem.
164
526-530
1987
Escherichia coli
Armstrong, F.B.; Lipscomb, E.L.; Crout, D.H.G.; Morgan, P.J.
Structure-activity studies with alpha,beta-dihydroxyacid dehydratase of Salmonella typhimurium
J. Chem. Soc. Perkin Trans.
1
691-696
1985
Salmonella enterica subsp. enterica serovar Typhimurium
-
Flint, D.H.; Tuminello, J.F.; Emptage, M.H.
The inactivation of Fe-S cluster containing hydro-lyases by superoxide
J. Biol. Chem.
268
22369-22376
1993
BRENDA: Escherichia coli
Textmining: Spinacia oleracea, Sus scrofa
Flint, D.H.; Emptage, M.H.; Finnegan, M.G.; Fu, W.; Johnson, M.K.
The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase
J. Biol. Chem.
268
14732-14742
1993
BRENDA: Escherichia coli
Textmining: Spinacia oleracea
Flint, D.H.; Smyk-Randall, E.; Tuminello, J.F.; Draczynska-Lusiak, B.; Brown, O.R.
The inactivation of dihydroxy-acid dehydratase in Escherichia coli treated with hyperbaric oxygen occurs because of the destruction of its Fe-S cluster, but the enzyme remains in the cell in a form that can be reactivated
J. Biol. Chem.
268
25547-25552
1993
Escherichia coli
Babu, B.N.; Brown, O.R.
Quantitative effects of redox-cycling chemicals on the oxidant-sensitive enzyme dihydroxy-acid dehydratase
Microbios
82
157-170
1995
BRENDA: Escherichia coli
Textmining: plant
Takabatake, T.; Hasegawa, M.; Nagano, T.; Hirobe, M.
Bacteriostatic effect of 4,7-dicyanobenzofurazan due to inactivation of 2,3-dihydroxyisovalerate dehydratase
Chem. Pharm. Bull.
40
1644-1646
1992
Escherichia coli
Matsumura, Y.; Imanaka, T.
Isolation and characterization of oxygen sensitive mutants of Escherichia coli
J. Ferment. Bioeng.
74
262-266
1992
Escherichia coli
-
Limberg, G.; Klaffke, W.; Thiem, J.
Conversion of aldonic acids to their corresponding 2-keto-3-deoxy-analogs by the non-carbohydrate enzyme dihydroxy acid dehydratase (DHAD)
Bioorg. Med. Chem.
3
487-494
1995
Spinacia oleracea
Flint, D.H.; Tuminello, J.F.; Miller, T.J.
Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis
J. Biol. Chem.
271
16053-16067
1996
Escherichia coli
Xing, R.; Whitman, W.B.
Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp
J. Bacteriol.
173
2086-2092
1991
BRENDA: Methanococcus aeolicus, Methanococcus maripaludis, Methanococcus voltae
Textmining: Methanococcus
Smyk-Randall, E.M.; Brown, O.R.
A reverse-phase high-performance liquid chromatography assay for dihydroxy-acid dehydratase
Anal. Biochem.
164
434-438
1987
Escherichia coli
Pirrung, M.C.; Holmes, C.P.; Horowitz, D.M.; Nunn, D.S.
Mechanism and stereochemistry of alpha,beta-dihydroxyacid dehydratase
J. Am. Chem. Soc.
113
1020-1025
1991
Spinacia oleracea
-
Leyval, D.; Uy, D.; Delaunay, S.; Goergen, J.L.; Engasser, J.M.
Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum
J. Biotechnol.
104
241-252
2003
Corynebacterium glutamicum
Kim, S.; Lee, S.B.
Catalytic promiscuity in dihydroxy-acid dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus
J. Biochem.
139
591-596
2006
BRENDA: Saccharolobus solfataricus (Q97UB2), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97UB2)
Textmining: archaeon, Bacteria, Escherichia coli, Archaea, eukaryota
Hyduke, D.R.; Jarboe, L.R.; Tran, L.M.; Chou, K.J.; Liao, J.C.
Integrated network analysis identifies nitric oxide response networks and dihydroxyacid dehydratase as a crucial target in Escherichia coli
Proc. Natl. Acad. Sci. USA
104
8484-8489
2007
BRENDA: Escherichia coli
Textmining: Mammalia, Bacteria
Duan, X.; Yang, J.; Ren, B.; Tan, G.; Ding, H.
Reactivity of nitric oxide with the [4Fe-4S] cluster of dihydroxyacid dehydratase from Escherichia coli
Biochem. J.
417
783-789
2009
BRENDA: Escherichia coli
Textmining: Bacteria
Holatko, J.; Elisakova, V.; Prouza, M.; Sobotka, M.; Nesvera, J.; Patek, M.
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation
J. Biotechnol.
139
203-210
2009
Corynebacterium glutamicum
Ren, B.; Zhang, N.; Yang, J.; Ding, H.
Nitric oxide-induced bacteriostasis and modification of iron-sulphur proteins in Escherichia coli
Mol. Microbiol.
70
953-964
2008
BRENDA: Escherichia coli
Textmining: Bacteria
Krause, F.S.; Blombach, B.; Eikmanns, B.J.
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production
Appl. Environ. Microbiol.
76
8053-8061
2010
Corynebacterium glutamicum
Li, S.; Wen, J.; Jia, X.
Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression
Appl. Microbiol. Biotechnol.
91
577-589
2011
BRENDA: Corynebacterium glutamicum (Q8NQZ9), Corynebacterium glutamicum
Textmining: Bacillus subtilis
Park, J.H.; Jang, Y.S.; Lee, J.W.; Lee, S.Y.
Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineering
Biotechnol. Bioeng.
108
1140-1147
2011
Escherichia coli
Denina, I.; Paegle, L.; Prouza, M.; Holatko, J.; Patek, M.; Nesvera, J.; Ruklisha, M.
Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC)
J. Ind. Microbiol. Biotechnol.
37
689-699
2010
Corynebacterium glutamicum (Q8NQZ9), Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 (Q8NQZ9)
Singh, V.; Chandra, D.; Srivastava, B.S.; Srivastava, R.
Downregulation of Rv0189c, encoding a dihydroxyacid dehydratase, affects growth of Mycobacterium tuberculosis in vitro and in mice
Microbiology
157
38-46
2011
BRENDA: Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Textmining: Mus sp., Mammalia
Oliver, J.D.; Kaye, S.J.; Tuckwell, D.; Johns, A.E.; Macdonald, D.A.; Livermore, J.; Warn, P.A.; Birch, M.; Bromley, M.J.
The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence
PLoS ONE
7
e43559
2012
BRENDA: Aspergillus fumigatus
Textmining: Bacteria, Fungi, Saccharomyces cerevisiae, Homo sapiens, Mus musculus
Carsten, J.M.; Philipp, A.; Sieber, V.
Characterization of recombinantly expressed dihydroxy-acid dehydratase from Sulfobus solfataricus - a key enzyme for the conversion of carbohydrates into chemicals
J. Biotechnol.
211
31-41
2015
BRENDA: Saccharolobus solfataricus (Q97UB2), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97UB2)
Textmining: Escherichia coli
Blombach, B.; Riester, T.; Wieschalka, S.; Ziert, C.; Youn, J.; Wendisch, V.; Eikmanns, B.
Corynebacterium glutamicum tailored for efficient isobutanol production
Appl. Environ. Microbiol.
77
3300-3310
2011
Corynebacterium glutamicum
Lee, W.; Seo, S.; Bae, Y.; Nan, H.; Jin, Y.; Seo, J.
Isobutanol production in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes
Bioprocess Biosyst. Eng.
35
1467-1475
2012
Saccharomyces cerevisiae, Saccharomyces cerevisiae (P07342)
Oh, B.R.; Heo, S.Y.; Lee, S.M.; Hong, W.K.; Park, J.M.; Jung, Y.R.; Kim, D.H.; Sohn, J.H.; Seo, J.W.; Kim, C.H.
Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain
Biotechnol. Lett.
36
57-62
2014
Klebsiella pneumoniae, Lactococcus lactis
Lu, J.; Brigham, C.J.; Plassmeier, J.K.; Sinskey, A.J.
Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16
Appl. Microbiol. Biotechnol.
99
761-774
2015
BRENDA: Cupriavidus necator (Q0K7F8), Cupriavidus necator
Textmining: Cupriavidus necator H16, Eutropha, Maniola tithonus
Gao, H.; Azam, T.; Randeniya, S.; Couturier, J.; Rouhier, N.; Johnson, M.K.
Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis
J. Biol. Chem.
293
4422-4433
2018
BRENDA: Arabidopsis thaliana (Q9LIR4)
Textmining: Bacteria, Fungi
Zhang, C.; Pang, Q.; Jiang, L.; Wang, S.; Yan, X.; Chen, S.; He, Y.
Dihydroxyacid dehydratase is important for gametophyte development and disruption causes increased susceptibility to salinity stress in Arabidopsis
J. Exp. Bot.
66
879-888
2015
BRENDA: Arabidopsis thaliana (Q9LIR4), Arabidopsis thaliana
Textmining: Arabidopsis, Bacteria, Fungi, Homo sapiens
Wu, C.; Lan, L.; Li, Y.; Nie, Z.; Zeng, R.
The relationship between latex metabolism gene expression with rubber yield and related traits in Hevea brasiliensis
BMC Genomics
19
897
2018
Hevea brasiliensis (A0A481UJA7), Hevea brasiliensis
Weitz, S.; Hermann, M.; Linder, S.; Bengelsdorf, F.; Takors, R.; Duerre, P.
Isobutanol production by autotrophic acetogenic bacteria
Front. Bioeng. Biotechnol.
9
657253
2021
Clostridium ljungdahlii
Flint, DH
Escherichia coli fumarase A catalyzes the isomerization of enol and keto oxalacetic acid.
Biochemistry
32
799-805
1993
Sus scrofa
Elliott, CJ; Armstrong, FB
Isoleucine-valine requiring mutants of Salmonella typhimurium. II. Strains deficient in dihydroxyacid dehydratase activity.
Genetics
58
171-9
1968
Salmonella enterica subsp. enterica serovar Typhimurium
Altmiller, DH; Wagner, RP
Deficiency of dihydroxy acid dehydratase in the mitochondria of the iv-I mutants of Neurospora crassa.
Biochem Genet
4
243-51
1970
Neurospora crassa
Kiritani, K; Narise, S; Wagner, RP
The dihydroxy acid dehydratase of Neurospora crassa.
J Biol Chem
241
2042-6
1966
Neurospora crassa
Brown, OR; Smyk-Randall, E; Draczynska-Lusiak, B; Fee, JA
Dihydroxy-acid dehydratase, a [4Fe-4S] cluster-containing enzyme in Escherichia coli: effects of intracellular superoxide dismutase on its inactivation by oxidant stress.
Arch Biochem Biophys
319
10-22
1995
Escherichia coli, Escherichia coli K-12, Candidatus Planktothricoides niger
Smyk-Randall, E; Brown, OR; Wilke, A; Eisenstark, A; Flint, DH
Near ultraviolet light inactivation of dihydroxyacid dehydratase in Escherichia coli.
Free Radic Biol Med
14
609-13
1993
Escherichia coli
Flint, DH
Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase.
J Biol Chem
271
16068-74
1996
Azotobacter vinelandii, Escherichia coli
WIXOM, RL; WIKMAN, JH
Search for a dihydroxy acid dehydrase in rat and microbial tissues.
Biochim Biophys Acta
45
618-9
1960
Rattus
KANAMORI, M; WIXOM, RL
Studies in valine biosynthesis. V. Characteristics of the purified dihydroxyacid dehydratase from spinach leaves.
J Biol Chem
238
998-1005
1963
Spinacia oleracea
SATYANARAYANA, T; RADHAKRISHNAN, AN
BIOSYNTHESIS OF VALINE AND ISOLEUCINE IN PLANTS. II. DIHYDROXYACID DEHYDRATASE FROM PHASEOLUS RADIATUS.
Biochim Biophys Acta
92
367-77
1964
Phaseolus, Vigna radiata var. radiata
Bashiri, G; Grove, TL; Hegde, SS; Lagautriere, T; Gerfen, GJ; Almo, SC; Squire, CJ; Blanchard, JS; Baker, EN
The active site of the Mycobacterium tuberculosis branched-chain amino acid biosynthesis enzyme dihydroxyacid dehydratase contains a 2Fe-2S cluster.
J Biol Chem
294
13158-13170
2019
Mycobacterium tuberculosis
Eprintsev, AT; Fedorin, DN; Anokhina, GB; Igamberdiev, AU
Effects of light, anoxia and salinity on the expression of dihydroxyacid dehydratase in maize.
J Plant Physiol
265
153507
2021
Zea mays, Anoxia
Schloss, JV
Oxygen toxicity from plants to people.
Planta
216
38-43
2002
Bacteria, Homo sapiens, Mammalia
Yang, Y; Zhang, Z; Lu, X; Gu, J; Wang, Y; Yao, Y; Liao, X; Shi, J; Lye, G; Baganz, F; Hao, J
Production of 2,3-dihydroxyisovalerate by Enterobacter cloacae.
Enzyme Microb Technol
140
109650
2020
Bacteria, Enterobacter cloacae, Klebsiella pneumoniae
Kochevenko, A; Fernie, AR
The genetic architecture of branched-chain amino acid accumulation in tomato fruits.
J Exp Bot
2011
plant, Solanum lycopersicum, Solanum pennellii
Park, YM; Lee, HJ; Jeong, JH; Kook, JK; Choy, HE; Hahn, TW; Bang, IS
Branched-chain amino acid supplementation promotes aerobic growth of Salmonella Typhimurium under nitrosative stress conditions.
Arch Microbiol
2015
Salmonella enterica subsp. enterica serovar Typhimurium
Zhang, H; Li, Y; Wang, X
[Metabolic engineering of L-valine synthesis and secretory pathways in Corynebacterium glutamicum for higher production].
Sheng Wu Gong Cheng Xue Bao
34
1606-1619
2018
Corynebacterium glutamicum
Tan, G; Lu, J; Bitoun, JP; Huang, H; Ding, H
IscA/SufA paralogs are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions.
Biochem J
2009
Escherichia coli
Tarleton, JC; Ely, B
Isolation and characterization of ilvA, ilvBN, and ilvD mutants of Caulobacter crescentus.
J Bacteriol
173
1259-67
1991
Caulobacter vibrioides, Bacteria, Escherichia coli, Serratia marcescens, plasmids, Isla Vista hantavirus
Seither, RL; Brown, OR
Paraquat and nitrofurantoin inhibit growth of Escherichia coli by inducing stringency.
J Toxicol Environ Health
14
763-71
1984
Escherichia coli
Velasco, JA; Cansado, J; Peña, MC; Kawakami, T; Laborda, J; Notario, V
Cloning of the dihydroxyacid dehydratase-encoding gene (ILV3) from Saccharomyces cerevisiae.
Gene
137
179-85
1993
yeasts, Saccharomyces cerevisiae, eukaryota, Isla Vista hantavirus
Radmacher, E; Vaitsikova, A; Burger, U; Krumbach, K; Sahm, H; Eggeling, L
Linking central metabolism with increased pathway flux: L-valine accumulation by Corynebacterium glutamicum.
Appl Environ Microbiol
68
2246-50
2002
Corynebacterium glutamicum
Vieites, JM; de la Torre, R; Ortega, MA; Montero, T; Peco, JM; Sánchez-Pozo, A; Gil, A; Suárez, A
Characterization of human cd200 glycoprotein receptor gene located on chromosome 3q12-13.
Gene
311
99-104
2003
Homo sapiens, insertion sequences, Mus musculus
Bckle-Vallant, V; Krause, FS; Messerschmidt, S; Eikmanns, BJ
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisocaproate production.
Appl Microbiol Biotechnol
98
297-311
2014
Corynebacterium glutamicum
Generoso, WC; Brinek, M; Dietz, H; Oreb, M; Boles, E
Secretion of 2,3-dihydroxyisovalerate as a limiting factor for isobutanol production in Saccharomyces cerevisiae.
FEMS Yeast Res
17
2017
Saccharomyces cerevisiae
Park, SH; Hahn, JS
Development of an efficient cytosolic isobutanol production pathway in Saccharomyces cerevisiae by optimizing copy numbers and expression of the pathway genes based on the toxic effect of ?-acetolactate.
Sci Rep
9
3996
2019
Saccharomyces cerevisiae, Bacillus subtilis
Liu, X; Jiang, Y; Zhang, Y; Yu, M; Jiang, H; Xu, J; Shi, J
FgIlv3a is crucial in branched-chain amino acid biosynthesis, vegetative differentiation, and virulence in Fusarium graminearum.
J Microbiol
57
694-703
2019
Fusarium graminearum, Bacteria, Fungi, Fusarium, Mammalia
Oh BR;Heo SY;Lee SM;Hong WK;Park JM;Jung YR;Kim DH;Sohn JH;Seo JW;Kim CH
Erratum to: Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain
Biotechnology letters
36
397-402
2014
Klebsiella pneumoniae, Lactococcus lactis
Oh BR;Heo SY;Lee SM;Hong WK;Park JM;Jung YR;Kim DH;Sohn JH;Seo JW;Kim CH
Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain
Biotechnology letters
36
57-62
2014
Klebsiella pneumoniae, Lactococcus lactis
Ren, B; Duan, X; Ding, H
Redox control of the DNA damage-inducible protein DinG helicase activity via its iron-sulfur cluster.
J Biol Chem
284
4829-35
2009
Escherichia coli
Muramatsu, R; Misawa, S; Hayashi, H
Finding of an isoleucine derivative of a recombinant protein for pharmaceutical use.
J Pharm Biomed Anal
31
979-87
2003
Escherichia coli
Miyanoiri, Y; Ishida, Y; Takeda, M; Terauchi, T; Inouye, M; Kainosho, M
Highly efficient residue-selective labeling with isotope-labeled Ile, Leu, and Val using a new auxotrophic E. coli strain.
J Biomol NMR
65
109-19
2016
Escherichia coli
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