Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 4.2.1.9 - dihydroxy-acid dehydratase and Organism(s) Corynebacterium glutamicum and UniProt Accession Q8NQZ9

for references in articles please use BRENDA:EC4.2.1.9
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.9 dihydroxy-acid dehydratase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Corynebacterium glutamicum
UNIPROT: Q8NQZ9 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Corynebacterium glutamicum
The enzyme appears in selected viruses and cellular organisms
Synonyms
dihydroxy-acid dehydratase, dihydroxyacid dehydratase, dihydroxy acid dehydratase, afilv3a, 2,3-dihydroxyisovalerate dehydratase, ssdhad, dihydroxy acid dehydrase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dihydroxyacid dehydratase
-
2,3-dihydroxy acid hydrolyase
-
-
-
-
2,3-dihydroxyisovalerate dehydratase
-
-
-
-
acetohydroxyacid dehydratase
-
-
-
-
alpha,beta-dihydroxy acid dehydratase
-
-
-
-
alpha,beta-dihydroxyisovalerate dehydratase
-
-
-
-
DAD
-
-
-
-
dehydratase, dihydroxy acid
-
-
-
-
dihydroxy acid dehydrase
-
-
-
-
dihydroxy acid dehydratase
-
-
-
-
dihydroxyacid dehydratase
VEG110
-
-
-
-
Vegetative protein 110
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxy-3-methylbutanoate hydro-lyase (3-methyl-2-oxobutanoate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9024-32-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxy-3-methylbutanoate
3-methyl-2-oxobutanoate + H2O
show the reaction diagram
2,3-dihydroxy-3-methylvalerate
3-methyl-2-oxovalerate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxy-isovalerate
2-oxoisovalerate
show the reaction diagram
-
-
-
-
?
DL-2,3-dihydroxyisovalerate
2-oxo-isovalerate + H2O
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxy-3-methylbutanoate
3-methyl-2-oxobutanoate + H2O
show the reaction diagram
2,3-dihydroxy-3-methylvalerate
3-methyl-2-oxovalerate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxy-isovalerate
2-oxoisovalerate
show the reaction diagram
-
-
-
-
?
DL-2,3-dihydroxyisovalerate
2-oxo-isovalerate + H2O
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ilvD
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
DHAD is involved in the biosynthetic pathways of L-valine, L-isoleucine, L-leucine, and D-pantothenate, regulation, overview
metabolism
-
dihydroxyacid dehydratase, a key enzyme involved in branched-chain amino acid biosynthesis, catalyses the synthesis of 2-oxoacids from dihydroxyacids
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ilvD, co-expression with other enzymes of the branched amino acid biosynthesis pathway in Bacillus subtilis strain 168 for production of isobutanol via the Ehrlich pathway, overview. Bacillus subtilis is engineered as the cell factory for isobutanol production due to its high tolerance to isobutanol. Subcloning in Escherichia coli strain JM109
gene ilvD, expression in Escherichia coli DH5alpha, overexpression
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Holatko, J.; Elisakova, V.; Prouza, M.; Sobotka, M.; Nesvera, J.; Patek, M.
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation
J. Biotechnol.
139
203-210
2009
Corynebacterium glutamicum
Manually annotated by BRENDA team
Krause, F.S.; Blombach, B.; Eikmanns, B.J.
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production
Appl. Environ. Microbiol.
76
8053-8061
2010
Corynebacterium glutamicum
Manually annotated by BRENDA team
Li, S.; Wen, J.; Jia, X.
Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression
Appl. Microbiol. Biotechnol.
91
577-589
2011
Corynebacterium glutamicum (Q8NQZ9), Corynebacterium glutamicum
Manually annotated by BRENDA team
Denina, I.; Paegle, L.; Prouza, M.; Holatko, J.; Patek, M.; Nesvera, J.; Ruklisha, M.
Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC)
J. Ind. Microbiol. Biotechnol.
37
689-699
2010
Corynebacterium glutamicum (Q8NQZ9), Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 (Q8NQZ9)
Manually annotated by BRENDA team