Information on EC 4.2.1.88 - synephrine dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.88
-
RECOMMENDED NAME
GeneOntology No.
synephrine dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-synephrine = (4-hydroxyphenyl)acetaldehyde + methylamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-O bond cleavage by elimination of water
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aromatic biogenic amine degradation (bacteria)
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-synephrine hydro-lyase (methylamine-forming)
Removal of H2O from (R)-synephrine produces a 2,3-enamine, which hydrolyses to the products shown in the reaction above. The enzyme from Arthrobacter synephrinum is highly specific [1].
CAS REGISTRY NUMBER
COMMENTARY hide
104118-54-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Arthrobacter synephrinum
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+/-)-octopamine
(4-hydroxyphenyl)acetaldehyde + NH3 + H2O
show the reaction diagram
Arthrobacter synephrinum
-
-
-
-
?
(+/-)-synephrine
4-hydroxyphenylacetaldehyde + methylamine
show the reaction diagram
(R)-synephrine
(4-hydroxyphenyl)acetaldehyde + methylamine
show the reaction diagram
Arthrobacter synephrinum
-
requires a sulfhydryl compound for activity, molecular oxygen is not utilized during the course of reaction
-
?
beta-methoxysynephrine
?
show the reaction diagram
Arthrobacter synephrinum
-
-
-
-
?
additional information
?
-
Arthrobacter synephrinum
-
not: tyramine, N-methyltyramine, N,N’-dimethyltyramine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+/-)-synephrine
4-hydroxyphenylacetaldehyde + methylamine
show the reaction diagram
Arthrobacter synephrinum
-
(+/-)-synephrine metabolism
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
Arthrobacter synephrinum
-
stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
Arthrobacter synephrinum
-
-
Cu2+
Arthrobacter synephrinum
-
-
denaturing agent
Arthrobacter synephrinum
-
-
-
Hg2+
Arthrobacter synephrinum
-
-
metal chelating agent
Arthrobacter synephrinum
-
-
-
thiol reagent
Arthrobacter synephrinum
-
-
-
Zn2+
Arthrobacter synephrinum
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Arthrobacter synephrinum
-
enhances activity
glutathione
Arthrobacter synephrinum
-
enhances activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.8
Arthrobacter synephrinum
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
Arthrobacter synephrinum
-
around
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Arthrobacter synephrinum
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
Arthrobacter synephrinum
-
4 * 42000
156000
Arthrobacter synephrinum
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
Arthrobacter synephrinum
-
4 * 42000
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
40fold
Arthrobacter synephrinum
-
partial
Arthrobacter synephrinum
-