Information on EC 4.2.1.85 - dimethylmaleate hydratase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eubacterium barkeri

EC NUMBER
COMMENTARY hide
4.2.1.85
-
RECOMMENDED NAME
GeneOntology No.
dimethylmaleate hydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2R,3S)-2,3-dimethylmalate = dimethylmaleate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-O bond cleavage by elimination of water
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
-
-
Nicotinate and nicotinamide metabolism
-
-
nicotinate degradation III
-
-
SYSTEMATIC NAME
IUBMB Comments
(2R,3S)-2,3-dimethylmalate hydro-lyase (dimethylmaleate-forming)
Requires Fe2+. Inhibited by oxygen.
CAS REGISTRY NUMBER
COMMENTARY hide
93229-56-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylmaleate + H2O
(2R,3S)-2,3-dimethylmalate
show the reaction diagram
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
required
additional information
-
Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
-
1 mM: 97% inhibition
EDTA
-
1-5 mM: 70% loss of activity
N-ethylmaleimide
-
1 mM: 93% inhibition
additional information
-
10 min, 25C, 2 mM iodoacetamide or iodoacetate: no influence
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
dimethylmaleate
-
35C, pH 7
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
broad
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
153000
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable during purification, probably because of dissociation into inactive protein compounds
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive
-
648458
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 45% loss of activity within 1 day
-
4C, 85% loss of activity within 1 day
-
liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATCC 25849, partial
-
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions
-
Show AA Sequence (286 entries)
Please use the Sequence Search for a specific query.