The enzyme is specific for (2S,3S)-methylcitrate, showing no activity with (2R,3S)-methylcitrate . The enzyme can also use cis-aconitate as a substrate but more slowly . Both this enzyme and EC 18.104.22.168, aconitate hydratase, are required to complete the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate .
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purified recombinant His-tagged enzyme, 11.7 mg/ml, in 10 mM HEPES, pH 7.5, optimization of crystallization conditions with precipitant solution containing PEG or pentaerythriol propoxylate and pentaerythriol ethoxylate, overview, X-ray diffraction structure determination and analysis at beyond 2.0 A resolution