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Information on EC 4.2.1.75 - uroporphyrinogen-III synthase and Organism(s) Arabidopsis thaliana and UniProt Accession O48721

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.75 uroporphyrinogen-III synthase
IUBMB Comments
In the presence of EC 2.5.1.61, hydroxymethylbilane synthase, the enzyme forms uroporphyrinogen III from porphobilinogen.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O48721
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
uros, uroporphyrinogen iii synthase, porphobilinogenase, uro-synthase, uroiiis, uroporphyrinogen iii cosynthase, u3s, uro synthase, uro'gen iii synthase, uroporphyrinogen-iii synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Uroporphyrinogen III synthase
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Hydroxymethylbilane hydrolyase [cyclizing]
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Isomerase, uroporphyrinogen
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Porphobilinogenase
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Synthase, uroporphyrinogen III co-
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URO-synthase
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UROIIIS
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Uroporphyrinogen III co-synthase
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Uroporphyrinogen III cosynthase
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Uroporphyrinogen III synthase
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Uroporphyrinogen isomerase
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Uroporphyrinogen-III cosynthase
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Uroporphyrinogen-III cosynthetase
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UROS
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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SYSTEMATIC NAME
IUBMB Comments
hydroxymethylbilane hydro-lyase (cyclizing; uroporphyrinogen-III-forming)
In the presence of EC 2.5.1.61, hydroxymethylbilane synthase, the enzyme forms uroporphyrinogen III from porphobilinogen.
CAS REGISTRY NUMBER
COMMENTARY hide
37340-55-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hydroxymethylbilane
uroporphyrinogen III + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hydroxymethylbilane
uroporphyrinogen III + H2O
show the reaction diagram
a step in tetrapyrrole biosynthesis, e.g. of chlorophyll, haem, sirohaem and bilins, overview
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
exclusive localization in chloroplasts, UROS has an N-terminal extension acting as a targeting sequence for import of the precursor protein of 34 kD into chloroplasts, followed by processing to the mature size of 29 kDa
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HEM4_ARATH
321
0
34225
Swiss-Prot
Chloroplast (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
x * 34000, precursor enzyme, x * 29000, mature enzyme
34000
x * 34000, precursor enzyme, x * 29000, mature enzyme
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 34000, precursor enzyme, x * 29000, mature enzyme
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
the N-terminal extension of UROS is processed resulting in the mature enzyme of 29 kDa
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene HEM4, library screening and complementation studies, sequence comparisons, expression of the enzyme in the knockout Saccharomyces cerevisiae S150-2B strain, overvexpression of the His-tagged enzyme in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tan, F.; Cheng, Q.; Saha, K.; Heinemann, I.U.; Jahn, M.; Jahn, D.; Smith, A.G.
Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase
Biochem. J.
410
291-299
2008
Arabidopsis thaliana (O48721), Arabidopsis thaliana
Manually annotated by BRENDA team