Information on EC 4.2.1.7 - altronate dehydratase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
4.2.1.7
-
RECOMMENDED NAME
GeneOntology No.
altronate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-altronate = 2-dehydro-3-deoxy-D-gluconate + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galacturonate degradation I
-
-
Metabolic pathways
-
-
Pentose and glucuronate interconversions
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-
degradation of sugar acids
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SYSTEMATIC NAME
IUBMB Comments
D-altronate hydro-lyase (2-dehydro-3-deoxy-D-gluconate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
62213-26-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K12
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-altronate
2-dehydro-3-deoxy-D-gluconate + H2O
show the reaction diagram
D-altronate
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-altronate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
activates, synergistic activation in presence of both, Mg2+ and Mn2+. Mn2+ appears to be a constituent of the enzyme active center
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
alpha-picolinate
-
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D-arabonate
-
competitive
D-glucarate
-
weak
Fe2+
-
low concentrations required, inhibition above 2 mM
iodoacetate
-
-
nitrilotriacetic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
sulfhydryl compounds required, 50 mM 2-mercaptoethanol in presence of 0.8 mM FeSO4 is optimal
Cys
-
increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
D-Altronate
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-
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.2
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.6
-
about 20% of maximal activity at pH 6.5 and at pH 8.6
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
-
x * 53000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
dialysis below pH 7 inactivates
33957
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59
-
half-life: 4 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of high concentrations, 0.1 M, of Cys or mercaptoethanol and maintenance in pH range pH 7.0-8.0 stabilizes throughout purification
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freezing, agitation or stirring in the presence of air or dialysis at pH levels below pH 7.0 inactivates
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sulfhydryl compounds stabilize
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-5°C, loss of activity after 2-3 weeks
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4°C, crude extract, enzyme retains 90% of its initial activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (809 entries)
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