Information on EC 4.2.1.69 - cyanamide hydratase

Word Map on EC 4.2.1.69
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.69
-
RECOMMENDED NAME
GeneOntology No.
cyanamide hydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
urea = cyanamide + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydration
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Atrazine degradation
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
urea hydro-lyase (cyanamide-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
50812-20-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
transgenic plants
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyanamide + H2O
?
show the reaction diagram
-
no constitutive enzyme, induced by addition of cyanamide to growth medium
-
-
-
cyanamide + H2O
urea
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cyanamide + H2O
?
show the reaction diagram
-
no constitutive enzyme, induced by addition of cyanamide to growth medium
-
-
-
cyanamide + H2O
urea
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
above 0.01 M
Cu2+
-
above 0.01 M
Fe2+
-
above 0.01 M
Mg2+
-
above 0.01 M
Mn2+
-
above 0.01 M
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0037
cyanamide
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
gene cassette with cyanamide hydratase (cah) marker gene for antifungal activity assays in transgenic plants, regeneration of transgenic plants achieved in selective media amended with 5 mM cyanamide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8.5
-
less than 50% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 65
-
less than 50% of maximal activity above and below
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
x * 25000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 25000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
stable at
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, strains BL 21 (DE3) and JM109, pCAMBIA 1300 vector used for construction of gene cassette for genetic transformation in Nicotiana tabacum, cyanamide hydratase (cah) used as a marker gene
-
the expression of the Cah enzyme in soybean leaves does not greatly affect the concentrations of any of the metabolites measured in unsprayed 1C and Jack plants grown under normal conditions or when placed in the dark for 24 h
-
transgenic soybean plants can be produced that express the fungal Cah gene and thus acquire resistance to toxic cyanamide. These plants are phenotypically normal and fertile indicating that expression of the Cah protein has no effect on soybean plant metabolism
-
wild-type cah and its mutants are cloned and overexpressed in pQE-60 Escherichia coli expression system. Western blot analysis confirms the production of 27700 Da cah enzyme by all the recombinants. Agrobacterium-mediated transformation is performed in Nicotiana tabaccum cv. Samsun plants by employing the leaf-disc method. The integration and expression of cah gene in transgenic plants are confirmed by polymerase chain reaction, Southern and Western blot analyses
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
due to its innate ability to convert cyanamide to urea and the broad-spectrum antimicrobial activity of cyanamide, the cah gene can be used to facilitate plant growth promotion and biocontrol of phytopathogens