Information on EC 4.2.1.68 - L-fuconate dehydratase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.1.68
-
RECOMMENDED NAME
GeneOntology No.
L-fuconate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
L-fuconate hydro-lyase (2-dehydro-3-deoxy-L-fuconate-forming)
Also acts, slowly, on D-arabinonate.
CAS REGISTRY NUMBER
COMMENTARY hide
37292-84-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
pv. campestris str. ATCC 33913
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-altronate
?
show the reaction diagram
-
-
-
-
?
D-arabinonate
?
show the reaction diagram
-
dehydration of D-arabinonate is initiated by abstraction of the 2-proton by Lys220
-
-
?
D-arabonate
2-keto-3-deoxy-D-arabonate + H2O
show the reaction diagram
-
-
-
?
D-ribonate
?
show the reaction diagram
-
dehydrations of D-ribonate is initiated by abstraction of the 2-proton by His351
-
-
?
L-fuconate
2-dehydro-3-deoxy-L-fuconate + H2O
show the reaction diagram
L-Fuconate
?
show the reaction diagram
L-galactonate
2-keto-3-deoxy-L-galactonate + H2O
show the reaction diagram
L-talonate
?
show the reaction diagram
-
dehydrations of L-talonate is initiated by abstraction of the 2-proton by His351
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Fuconate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required for activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-arabonate
-
10 mM, 100% inhibition
D-arabono-1,5-lactone
-
-
D-galactonate
-
10 mM, 100% inhibition
D-gluconate
-
10 mM, 100% inhibition
EDTA
-
10 mM, 100% inhibition
HgCl2
-
0.25 mM, ca. 90% inhibition
iodoacetate
-
2.5 mM, 90% inhibition
L-Rhamnonate
-
10 mM, 95% inhibition
N-ethylmaleimide
-
1 mM, 81% inhibition
p-chloromercuribenzoate
-
0.25 mM, 79% inhibition
p-hydroxymercuribenzoate
-
-
ZnSO4
-
5 mM, 78% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
D-Arabinonate
-
25C, pH 7.5
1.3
L-arabonate
-
-
0.33 - 1
L-fuconate
3.3 - 6.2
L-galactonate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.4
D-Arabinonate
Xanthomonas campestris
-
25C, pH 7.5
15
L-fuconate
Xanthomonas campestris
-
25C, pH 7.5
0.9
L-galactonate
Xanthomonas campestris
-
25C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.066
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
less than 50% of maximal activity above and below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
less than 50% of maximal activity above and below
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
poor source
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95000
-
gel filtration
104000
-
sucrose density ultracentifugation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very unstable, not stabilized by MgCl2 or EDTA
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 30% loss of activity in 1 month
-
-60C, 5 mM dithiothreitol, 2mg/ml protein, stable for 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (106 entries)
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