Information on EC 4.2.1.67 - D-fuconate dehydratase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY hide
4.2.1.67
-
RECOMMENDED NAME
GeneOntology No.
D-fuconate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-Fuconate = 2-dehydro-3-deoxy-D-fuconate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-fuconate hydro-lyase (2-dehydro-3-deoxy-D-fuconate-forming)
Also acts on L-arabinonate.
CAS REGISTRY NUMBER
COMMENTARY hide
9076-59-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Fuconate
2-Dehydro-3-deoxy-D-fuconate + H2O
show the reaction diagram
L-Arabonate
2-Keto-3-deoxy-L-arabonate + H2O
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
optimum: 20 mM, required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CoCl2
-
13.3 mM, 18% residual activity
CuSO4
-
13.3 mM, 29% residual activity
FeSO4
-
13.3 mM, 37% residual activity
NiCl2
-
13.3 mM, 8% residual activity
ZnCl2
-
13.3 mM, complete inactivation
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
2.8 mM, activation
dithiothreitol
-
2.8 mM, activation
GSH
-
2.8 mM, activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
D-fuconate
-
-
4.3
L-arabonate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 7.4
-
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, 10% loss of activity after 1 week
-
frozen, 24 h, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE