Information on EC 4.2.1.65 - 3-Cyanoalanine hydratase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.2.1.65
-
RECOMMENDED NAME
GeneOntology No.
3-Cyanoalanine hydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-Asparagine = 3-cyanoalanine + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
-
-
-
-
hydrolysis
-
-
of nitrile group
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
cyanide detoxification I
-
-
Cyanoamino acid metabolism
-
-
Metabolic pathways
-
-
NIL
-
-
SYSTEMATIC NAME
IUBMB Comments
L-asparagine hydro-lyase (3-cyanoalanine-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37259-64-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
13
-
-
Manually annotated by BRENDA team
13
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-Cyano-L-alanine + H2O
?
show the reaction diagram
beta-Cyano-L-alanine + H2O
L-Asparagine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-Cyano-L-alanine + H2O
?
show the reaction diagram
beta-Cyano-L-alanine + H2O
L-Asparagine
show the reaction diagram
Q5QGZ8
enzyme involved in the cyanide detoxification pathway
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
-
poor
Ag+
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Cd2+
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
Cu2+
-
the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol
HgCl2
iodoacetic acid
Mersalyl acid
-
-
N-ethylmaleimide
NaF
-
poor
NaNO2
-
poor
p-chloromercuribenzoate
-
-
p-Chloromercuriphenyl sulfonic acid
-
-
Zn(CH3COO)2
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 83
Beta-cyano-L-alanine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 65
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
x * 35000, SDS-PAGE
400000 - 500000
-
gel filtration
1000000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
-
in presence of 2-mercaptoethanol
5824
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
up to, 10 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
not stabilized by rapid freezing in liquid nitrogen, storage at 4 or -20¦C with mercaptoethanol, dithiothreitol, beta-cyano-L-alanine or asparagine
-
stabilized as ammonium sulfate precipitate
-
very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10% glycerol, 10-15% loss of activity after 5 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli