Information on EC 4.2.1.62 - 5alpha-Hydroxysteroid dehydratase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY
4.2.1.62
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RECOMMENDED NAME
GeneOntology No.
5alpha-Hydroxysteroid dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
5alpha-Ergosta-7,22-diene-3beta,5-diol = ergosterol + H2O
show the reaction diagram
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-
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5alpha-Ergosta-7,22-diene-3beta,5-diol = ergosterol + H2O
show the reaction diagram
mechanism
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
elimination
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
5alpha-ergosta-7,22-diene-3beta,5-diol 5,6-hydro-lyase (ergosterol-forming)
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5alpha-Hydroxysterol dehydase
-
-
-
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Dehydratase, 5alpha-hydroxysterol
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
9075-27-8
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5alpha,8alpha-Epidioxyergosta-6,22-diene-3beta-ol
Ergosterol + H2O
show the reaction diagram
-
-
-
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5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
Ergosterol + H2O
show the reaction diagram
-
-
-
-
5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
Ergosterol + H2O
show the reaction diagram
-
-
-
-
5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
Ergosterol + H2O
show the reaction diagram
-
-
-
-
5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
?
show the reaction diagram
-
sterol biosynthesis
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
?
show the reaction diagram
-
sterol biosynthesis
-
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no requirement
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.17
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5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
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-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
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enzyme assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
enzyme assay at
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
side-chain modification
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phospholipid associated with active enzyme, removal by digestion with phospholipase A does not reduce the activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 0.4 M potassium phosphate buffer, pH 7.4, 10 mM 2-mercaptoethanol, 2 weeks, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE