Information on EC 4.2.1.6 - galactonate dehydratase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.1.6
-
RECOMMENDED NAME
GeneOntology No.
galactonate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galactonate degradation
-
-
degradation of sugar acids
-
-
Galactose metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
D-galactonate hydro-lyase (2-dehydro-3-deoxy-D-galactonate-forming)
The enzyme shows no activity with D-gluconate [2]. cf. EC 4.2.1.140, gluconate/galactonate dehydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-38-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
K10
UniProt
Manually annotated by BRENDA team
strain PRL-R3
-
-
Manually annotated by BRENDA team
strain PRL-R3
-
-
Manually annotated by BRENDA team
Mycobacterium butyricum
-
-
-
Manually annotated by BRENDA team
Mycobacterium friburgensis
-
-
-
Manually annotated by BRENDA team
Mycobacterium jucho
-
-
-
Manually annotated by BRENDA team
strain 279
-
-
Manually annotated by BRENDA team
strain 607
-
-
Manually annotated by BRENDA team
no activity in Botrytis cinerea
-
-
-
Manually annotated by BRENDA team
ATCC 27855
-
-
Manually annotated by BRENDA team
L5-30; UR23
-
-
Manually annotated by BRENDA team
L5-30
-
-
Manually annotated by BRENDA team
UR23
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-fuconate
2-keto-3-deoxy-D-fuconate + H2O
show the reaction diagram
D-galactonate
2-dehydro-3-deoxy-D-galactonate + H2O
show the reaction diagram
D-galactonate
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-galactonate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+
Ni2+
-
divalent cation required, in the order of decreasing effectiveness: Mg2+, Mn2+, Co2+, Ni2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CdSO4
Mycobacterium butyricum
-
-
CuSO4
Mycobacterium butyricum
-
-
HgCl2
Mycobacterium butyricum
-
-
iodoacetamide
-
-
iodoacetate
Mycobacterium butyricum
-
-
NaF
Mycobacterium butyricum
-
-
ZnSO4
Mycobacterium butyricum
-
reactivated by chelating agents
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3
D-fuconate
-
-
0.9 - 21.74
D-galactonate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.56
Mycobacterium butyricum
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 8
Mycobacterium butyricum
-
in 100 mM tris-HCl buffer or 50 mM phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 8
-
pH 5.9: about 40% of maximal activity, pH 8.0: about 25% of maximal activity
6 - 9
-
about 25% of maximal activity at pH 6.0 and at pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
about 45% of maximal activity at 20C and 60C
PDB
SCOP
CATH
ORGANISM
UNIPROT
Ralstonia pickettii (strain 12J)
Ralstonia pickettii (strain 12J)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000 - 245000
270000
290000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53
-
half-life: more than 1 h
60
-
5 min, 6% loss of activity, 60 min, 30% loss of activity
75
Mycobacterium butyricum
-
10 min, about 60% loss of activity. 15 min, about 90% loss of activity
81
-
half-life: 10 min
95
-
half-life: 3 min
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
insensitive to repeated freeze-thaw cycles
-
stable to lyophilization in 0.05 M glycine/0.01 M sodium cacodylate, pH 8.8
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 0.05 M glycine-0.01 M sodium cacodylate, pH 8.8, stable for 6 months
-
-20C, 6 months, stable
-
4C or -20C, stable for at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae
-
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