Information on EC 4.2.1.55 - 3-hydroxybutyryl-CoA dehydratase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
4.2.1.55
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RECOMMENDED NAME
GeneOntology No.
3-hydroxybutyryl-CoA dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
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elimination
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acetyl-CoA fermentation to butanoate II
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ethylmalonyl-CoA pathway
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butanoate fermentation
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CO2 fixation in Crenarchaeota
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tryptophan metabolism
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Glyoxylate and dicarboxylate metabolism
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Butanoate metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(3R)-3-hydroxybutanoyl-CoA hydro-lyase (crotonoyl-CoA-forming)
Also acts on crotonoyl thioesters of pantetheine and acyl-carrier protein.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-82-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3R)-3-hydroxybutanoyl-CoA
crotonoyl-CoA + H2O
show the reaction diagram
(3R)-3-Hydroxybutanoyl-CoA
Crotonyl-CoA + H2O
show the reaction diagram
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crotonoyl-CoA + H2O
(3R)-3-hydroxybutanoyl-CoA
show the reaction diagram
crotonyl-CoA
?
show the reaction diagram
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enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria
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Crotonyl-CoA + H2O
(3R)-3-Hydroxybutanoyl-CoA
show the reaction diagram
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Crotonyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
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about 50% of the activity with crotonyl-CoA
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Crotonylpantetheine
?
show the reaction diagram
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about 55% of the activity with crotonyl-CoA
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trans-2-Hexenoyl-CoA + H2O
?
show the reaction diagram
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about 35% of the activity with crotonyl-CoA
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3R)-3-hydroxybutanoyl-CoA
crotonoyl-CoA + H2O
show the reaction diagram
crotonoyl-CoA + H2O
(3R)-3-hydroxybutanoyl-CoA
show the reaction diagram
crotonyl-CoA
?
show the reaction diagram
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enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria
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additional information
?
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2-enoyl-CoA hydratase 2 is the middle part of the mammalian multifunctional protein-2, MFP-2, which catalyzes the (R)-specific hydration of 2-enoyl-CoA thioesters
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
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0.01 M, 30% inhibition
iodoacetic acid
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0.01 M, 12% inhibition
N-ethylmaleimide
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0.01 M, 23% inhibition
oct-2-yn-4-enoyl-CoA
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a multifunctional irreversible enzyme inhibitor in fatty acid oxidation mainly targeting mitochondrial trifunctional protein beta-subunit, alsoirreversibly inactivates enoyl-CoA hydratase 2, mechanism for inactivation, overview
p-Substituted mercuribenzoate
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3 mM, 65% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00926
crotonyl-CoA
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0.08
crotonyl-[acyl-carrier protein]
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0.118
Crotonylpantetheine
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0.0263
trans-2-hexenoyl-CoA
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
oct-2-yn-4-enoyl-CoA
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ECH 2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
85C, pH 7.0, highly enriched enzyme
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Myxococcus xanthus (strain DK 1622)
Myxococcus xanthus (strain DK 1622)
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
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2-enoyl-CoA hydratase 2 is the middle part of the MFP-2, the enzyme is a homodimer, unlike the mitochondrial 2-enoyl-CoA hydratase 1 that is a hexamer
homodimer
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2-enoyl-CoA hydratase 2 is the middle part of the MFP-2, the enzyme is a homodimer, unlike the mitochondrial 2-enoyl-CoA hydratase 1 that is a hexamer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Ralstonia eutropha cells
expression in Escherichia coli
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