Information on EC 4.2.1.55 - 3-Hydroxybutyryl-CoA dehydratase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY
4.2.1.55
-
RECOMMENDED NAME
GeneOntology No.
3-Hydroxybutyryl-CoA dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O
show the reaction diagram
stereochemistry
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
addition
-
-
-
-
elimination
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acetyl-CoA fermentation to butyrate II
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butanoate fermentation
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Butanoate metabolism
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CO2 fixation in Crenarchaeota
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ethylmalonyl pathway
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Glyoxylate and dicarboxylate metabolism
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Microbial metabolism in diverse environments
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tryptophan metabolism
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SYSTEMATIC NAME
IUBMB Comments
(3R)-3-hydroxybutanoyl-CoA hydro-lyase (crotonoyl-CoA-forming)
Also acts on crotonoyl thioesters of pantetheine and acyl-carrier protein.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
(R)-specific enoyl-CoA hydratase
-
-
CP 24
-
-
-
-
crotonase
-
-
-
-
D-3-Hydroxybutyryl coenzyme A dehydratase
-
-
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D-3-Hydroxybutyryl-CoA dehydratase
-
-
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Dehydratase, D-3-hydroxybutyryl coenzyme A
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-
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Enoyl coenzyme A hydrase (D)
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-
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enoyl coenzyme A hydratase
-
-
enoyl-CoA hydratase 2
-
-
Ferp_1035
D3RXI4
gene name. Bifunctional crotonase/3-OH-butyryl-CoA dehydrogenase
Ferp_1035
D3RXI4
gene name. Bifunctional crotonase/3-OH-butyryl-CoA dehydrogenase
-
CAS REGISTRY NUMBER
COMMENTARY
37290-82-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
D3RXI4
the enzyme is involved in benzoyl-coenzyme A degradation
metabolism
-
the enzyme is involved in benzoyl-coenzyme A degradation
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3R)-3-Hydroxybutanoyl-CoA
Crotonyl-CoA + H2O
show the reaction diagram
-
-
-
-
(3R)-3-hydroxybutanoyl-CoA
crotonoyl-CoA + H2O
show the reaction diagram
-
-
-
-
?
crotonoyl-CoA + H2O
(3R)-3-hydroxybutanoyl-CoA
show the reaction diagram
D3RXI4
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
crotonoyl-CoA + H2O
(3R)-3-hydroxybutanoyl-CoA
show the reaction diagram
D3RXI4
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
crotonyl-CoA
?
show the reaction diagram
-
enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria
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-
-
Crotonyl-CoA + H2O
(3R)-3-Hydroxybutanoyl-CoA
show the reaction diagram
-
-
-
-
Crotonyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
about 50% of the activity with crotonyl-CoA
-
-
-
trans-2-Hexenoyl-CoA + H2O
?
show the reaction diagram
-
about 35% of the activity with crotonyl-CoA
-
-
-
Crotonylpantetheine
?
show the reaction diagram
-
about 55% of the activity with crotonyl-CoA
-
-
-
additional information
?
-
-
no activity with crotonylglutathione, acrylyl-CoA
-
-
-
additional information
?
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2-enoyl-CoA hydratase 2 is the middle part of the mammalian multifunctional protein-2, MFP-2, which catalyzes the (R)-specific hydration of 2-enoyl-CoA thioesters
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3R)-3-hydroxybutanoyl-CoA
crotonoyl-CoA + H2O
show the reaction diagram
-
-
-
-
?
crotonoyl-CoA + H2O
(3R)-3-hydroxybutanoyl-CoA
show the reaction diagram
D3RXI4
the enzyme is involved in benzoyl-coenzyme A degradation
-
-
?
crotonyl-CoA
?
show the reaction diagram
-
enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria
-
-
-
additional information
?
-
-
2-enoyl-CoA hydratase 2 is the middle part of the mammalian multifunctional protein-2, MFP-2, which catalyzes the (R)-specific hydration of 2-enoyl-CoA thioesters
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
iodoacetamide
-
0.01 M, 30% inhibition
iodoacetic acid
-
0.01 M, 12% inhibition
N-ethylmaleimide
-
0.01 M, 23% inhibition
oct-2-yn-4-enoyl-CoA
-
a multifunctional irreversible enzyme inhibitor in fatty acid oxidation mainly targeting mitochondrial trifunctional protein beta-subunit, alsoirreversibly inactivates enoyl-CoA hydratase 2, mechanism for inactivation, overview
p-Substituted mercuribenzoate
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3 mM, 65% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00926
crotonyl-CoA
-
-
0.08
crotonyl-[acyl-carrier protein]
-
-
0.118
Crotonylpantetheine
-
-
0.0263
trans-2-Hexenoyl-CoA
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.025
oct-2-yn-4-enoyl-CoA
-
ECH 2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.7
D3RXI4
85°C, pH 7.0, highly enriched enzyme
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.4 - 8.6
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
85
D3RXI4
assay at
PDB
SCOP
CATH
ORGANISM
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexamer
-
2-enoyl-CoA hydratase 2 is the middle part of the MFP-2, the enzyme is a homodimer, unlike the mitochondrial 2-enoyl-CoA hydratase 1 that is a hexamer
homodimer
-
2-enoyl-CoA hydratase 2 is the middle part of the MFP-2, the enzyme is a homodimer, unlike the mitochondrial 2-enoyl-CoA hydratase 1 that is a hexamer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Ralstonia eutropha cells
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expression in Escherichia coli
D3RXI4
expressed in Ralstonia eutropha cells
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