Information on EC 4.2.1.54 - lactoyl-CoA dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.54
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RECOMMENDED NAME
GeneOntology No.
lactoyl-CoA dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-lactoyl-CoA = acryloyl-CoA + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
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-
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elimination
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Microbial metabolism in diverse environments
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Propanoate metabolism
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pyruvate fermentation to propanoate II (acrylate pathway)
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Styrene degradation
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alanine metabolism
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SYSTEMATIC NAME
IUBMB Comments
(R)-lactoyl-CoA hydro-lyase (acryloyl-CoA-forming)
A bacterial enzyme that is involved in propanoate fermentation (also known as the acrylate pathway).
CAS REGISTRY NUMBER
COMMENTARY hide
9031-12-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-2-hydroxybutyryl-CoA
crotonyl-CoA + H2O
show the reaction diagram
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stereospecific elimination of the 3Si hydrogen
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(R)-lactoyl-CoA
acryloyl-CoA + H2O
show the reaction diagram
acrylyl-CoA + H2O
lactoyl-CoA
show the reaction diagram
crotonoyl-CoA + H2O
2-hydroxybutyryl-CoA
show the reaction diagram
lactoyl-CoA
acrylyl-CoA
show the reaction diagram
lactoyl-CoA + H2O
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-lactoyl-CoA
acryloyl-CoA + H2O
show the reaction diagram
lactoyl-CoA + H2O
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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required, no stoichiometric hydrolysis of ATP
flavin
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component EII contains flavin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe-S
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component EII contains two Fe-S clusters one 4Fe-4S type, one 3Fe-3/4S type, the loss of Fe does not affect catalytic activity
Mg2+
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required
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.031
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recombinant enzyme, pH and temperature not specified in the publication
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
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enzyme consists of two proteins, EI and EII, neither of the two can catalyze the reaction alone, EI: 27000, EII: x * 41000 + x * 48000, SDS-PAGE
48000
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enzyme consists of two proteins, EI and EII, neither of the two can catalyze the reaction alone, EI: 27000, EII: x * 41000 + x * 48000, SDS-PAGE
1000000
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enzyme consists of two proteins, EI and EII, neither of the two can catalyze the reaction alone, EI: 27000 Da, EII: 10000000 Da, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
polymer
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enzyme consists of two proteins, EI and EII, neither of the two can catalyze the reaction alone, EI: 27000, EII: x * 41000 + x * 48000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very unstable during purification
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
exposure to O2 for 24 h causes complete inactivation
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33929
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, crude extract relatively stable
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4°C, anaerobic storage, 4 mM (NH4)2SO4, stable for at least 4 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta-gami 2(DE3)pLysS cells
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