Information on EC 4.2.1.50 - Pyrazolylalanine synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.50
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RECOMMENDED NAME
GeneOntology No.
Pyrazolylalanine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-serine + pyrazole = 3-(pyrazol-1-yl)-L-alanine + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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SYSTEMATIC NAME
IUBMB Comments
L-serine hydro-lyase [adding pyrazole; 3-(pyrazol-1-yl)-L-alanine-forming]
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-81-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
melon
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Manually annotated by BRENDA team
cucumber
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-
Manually annotated by BRENDA team
pumpkin, marrow
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + H2O
show the reaction diagram
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-
-
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L-Serine + pyrazole
?
show the reaction diagram
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-
-
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O-Acetyl-L-serine + 3-amino-1,2,4-triazole
?
show the reaction diagram
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10.6% of activity with pyrazole
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-
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O-Acetyl-L-serine + H2S
Cysteine + acetate
show the reaction diagram
-
-
-
-
O-Acetyl-L-serine + N-hydroxyurea
?
show the reaction diagram
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8.7% of activity with pyrazole
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-
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O-Acetyl-L-serine + pyrazole
3-(Pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
O-Acetyl-L-serine + pyrazole
?
show the reaction diagram
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-
-
-
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O-Succinyl-L-serine + pyrazole
?
show the reaction diagram
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16.4% of activity with O-Acetyl-L-serine
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-
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O-Sulfo-L-serine + pyrazole
?
show the reaction diagram
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2.5% of activity with O-Acetyl-L-serine
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additional information
?
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no activity with L-serine
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Serine + pyrazole
?
show the reaction diagram
-
-
-
-
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O-Acetyl-L-serine + pyrazole
?
show the reaction diagram
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-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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1 molecule bound to each subunit; maximal activation by 40% at 0.25 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
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50 mM almost complete inhibition
potassium cyanide
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10 mM almost complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
O-acetyl-L-serine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
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L-serine + pyrazole
7.3 - 7.4
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O-acetyl-L-serine + pyrazole
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1 - 7.7
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38% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.1 and 56% of maximal activity at pH 7.7
6.5 - 8.5
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50% of maximal activity with O-acetyl-L-serine + pyrazole at pH 6.5 and pH 8.5
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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2 * 32000, SDS-PAGE
58000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 32000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to freezing even in 20% glycerol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
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production of beta-(pyrazol-1-yl)-L-alanine for pharmacological use by enzyme overexpressed in E. coli