Information on EC 4.2.1.45 - CDP-glucose 4,6-dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.45
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RECOMMENDED NAME
GeneOntology No.
CDP-glucose 4,6-dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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-
-
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intramolecular oxido-reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
CDP-glucose 4,6-hydro-lyase (CDP-4-dehydro-6-deoxy-D-glucose-forming)
Requires bound NAD+.
CAS REGISTRY NUMBER
COMMENTARY hide
37259-55-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-D-glucose
CDP-4-dehydro-6-deoxy-D-glucose + H2O
show the reaction diagram
CDP-glucose
?
show the reaction diagram
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biosynthetic pathway of 3,6-dideoxysugars
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-
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dCDP-D-glucose
dCDP-4-dehydro-6-deoxy-D-glucose + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-glucose
?
show the reaction diagram
-
biosynthetic pathway of 3,6-dideoxysugars
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
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20% of the activity with NAD+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CDP-4-keto-6-deoxy-D-glucose
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-
CDP-6-deoxy-6,6-difluoro-D-glucose
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Ki: 0.94 mM
p-Chloromercuriphenylsulfonate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00015 - 0.4
CDP-D-glucose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.33 - 4.24
CDP-D-glucose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.94
CDP-6-deoxy-6,6-difluoro-D-glucose
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.78
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mutant H17G
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40280
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2 * 40280, electrospray ionization MS
42500
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2 * 42500, SDS-PAGE
43000
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2 * 43000, SDS-PAGE
70000 - 100000
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gel filtration
86000
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gel filtration
160000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallization of selenomethionine-labeled CDP-D-glucose 4,5-dehydratase and of selenomethionine-labeled CDP-D-glucose 4,5-dehydratase complexed with the substrate analog CDP-D-xylose, hanging-drop vapor diffusion, batch method at room temperature
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with NAD+, hanging drop vapor diffusion method
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
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not stable below
437994
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable to freezing in all stages of purification
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unstable in dilute salt solutions
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unstable to intermittant freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.02 M phosphate buffer, pH 7.5, several months, stable
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-20°C, several months, no loss of activity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H17G
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mutant with increased kcat and reduced Km
K21I
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mutant with reduced Km and kcat
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