Information on EC 4.2.1.44 - Myo-inosose-2 dehydratase

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The expected taxonomic range for this enzyme is: Enterobacter aerogenes

EC NUMBER
COMMENTARY
4.2.1.44
-
RECOMMENDED NAME
GeneOntology No.
Myo-inosose-2 dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2,4,6/3,5-Pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O
show the reaction diagram
-
-
-
-
2,4,6/3,5-Pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
elimination
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Inositol phosphate metabolism
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
myo-inositol degradation I
-
SYSTEMATIC NAME
IUBMB Comments
2,4,6/3,5-pentahydroxycyclohexanone hydro-lyase (3,5/4-trihydroxycyclohexa-1,2-dione-forming)
Requires Co2+ or Mn2+.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Dehydratase, inosose 2, 3-
-
-
-
-
Inosose 2,3-dehydratase
-
-
-
-
ketoinositol dehydratase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37290-79-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone
(3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
show the reaction diagram
-
-
-
-
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone
(3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
show the reaction diagram
-
pathway of myo-inositol degradation
-
-
-
L-2-Keto-epi-inositol
?
show the reaction diagram
-
-
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone
(3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O
show the reaction diagram
-
pathway of myo-inositol degradation
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
increases activity
Mn2+
-
increases activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
inhibition can be alleviated by either Mn2+ or Co2+
p-hydroxymercuribenzoate
-
on addition of glutathione or mercaptoethanol the activity is regained
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.15
-
2-keto-myo-inositol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
6.2
-
2-keto-myo-inositol
PDB
SCOP
CATH
ORGANISM
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
unstable when exposed to oxygen
-
5706
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-10°C, nitrogen atmosphere, for 1 month, about 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE