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Information on EC 4.2.1.41 - 5-dehydro-4-deoxyglucarate dehydratase and Organism(s) Acinetobacter baylyi and UniProt Accession Q6FFQ1

for references in articles please use BRENDA:EC4.2.1.41
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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.41 5-dehydro-4-deoxyglucarate dehydratase
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This record set is specific for:
Acinetobacter baylyi
UNIPROT: Q6FFQ1 not found.
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Word Map
The taxonomic range for the selected organisms is: Acinetobacter baylyi
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
kdg dehydratase, d-5-keto-4-deoxyglucarate dehydratase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-dehydro-4-deoxyglucarate dehydratase
-
5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase
-
D-5-keto-4-deoxyglucarate dehydratase
-
KDG dehydratase
-
4-Deoxy-5-oxoglucarate hydro-lyase (decarboxylating)
-
-
-
-
5-Keto-4-deoxy-glucarate dehydratase
-
-
-
-
D-4-Deoxy-5-ketoglucarate hydro-lyase
-
-
-
-
D-4-Deoxy-5-ketoglucarate hydro-lyase (decarboxylating)
-
-
-
-
D-4-Deoxy-5-oxoglucarate hydro-lyase (decarboxylating)
-
-
-
-
Dehydratase, deoxyketoglucarate
-
-
-
-
Deoxyketoglucarate dehydratase
-
-
-
-
Deoxyoxoglucarate dehydratase
-
-
-
-
KDGDH
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
5-dehydro-4-deoxy-D-glucarate hydro-lyase (decarboxylating; 2,5-dioxopentanoate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37290-77-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-dehydro-4-deoxy-D-glucarate
2,5-dioxopentanoate + H2O + CO2
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-dehydro-4-deoxy-D-glucarate
2,5-dioxopentanoate + H2O + CO2
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.203 - 1
5-dehydro-4-deoxy-D-glucarate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.9 - 4.5
5-dehydro-4-deoxy-D-glucarate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5
5-dehydro-4-deoxy-D-glucarate
pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
determined by SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
t1/2: 2.71 h in presence of 0.5 mM NH4HCO3
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
NH4HCO3 at a concentration of 0.5 mM, maintains stability of the enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli ArcticExpress(DE3)
into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
by establishing a pH-shift assay, an easy measurement method for 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylase is developed. It can be used for measurements of the purified enzymes or using crude extracts. It is especially suitable as the method of choice within an engineering approach for further optimization of the enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Aghaie, A.; Lechaplais, C.; Sirven, P.; Tricot, S.; Besnard-Gonnet, M.; Muselet, D.; de Berardinis, V.; Kreimeyer, A.; Gyapay, G.; Salanoubat, M.; Perret, A.
New Insights into the Alternative D-Glucarate Degradation Pathway
J. Biol. Chem.
283
15638-15646
2008
Acinetobacter baylyi (Q6FFQ1)
Manually annotated by BRENDA team
Pick, A.; Beer, B.; Hemmi, R.; Momma, R.; Schmid, J.; Miyamoto, K.; Sieber, V.
Identification and characterization of two new 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylases
BMC Biotechnol.
16
80
2016
Acinetobacter baylyi (Q6FFQ1), Acinetobacter baylyi, Comamonas testosteroni (B7WZA5), Comamonas testosteroni, Comamonas testosteroni KF-1 (B7WZA5), Comamonas testosteroni KF-1, Polaromonas naphthalenivorans (A1VMN2), Polaromonas naphthalenivorans, Polaromonas naphthalenivorans CJ2 (A1VMN2), Polaromonas naphthalenivorans CJ2
Manually annotated by BRENDA team