Information on EC 4.2.1.41 - 5-dehydro-4-deoxyglucarate dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.41
-
RECOMMENDED NAME
GeneOntology No.
5-dehydro-4-deoxyglucarate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-Dehydro-4-deoxy-D-glucarate = 2,5-dioxopentanoate + H2O + CO2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
-
-
D-galactarate degradation II
-
-
D-galacturonate degradation II
-
-
D-glucarate degradation II
-
-
D-glucuronate degradation II
-
-
Metabolic pathways
-
-
degradation of sugar acids
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-
SYSTEMATIC NAME
IUBMB Comments
5-dehydro-4-deoxy-D-glucarate hydro-lyase (decarboxylating; 2,5-dioxopentanoate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37290-77-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-Dehydro-4-deoxy-D-glucarate
2,5-Dioxopentanoate + H2O + CO2
show the reaction diagram
5-Dehydro-4-deoxy-D-glucarate
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-Dehydro-4-deoxy-D-glucarate
2,5-Dioxopentanoate + H2O + CO2
show the reaction diagram
Q6FFQ1
-
-
-
?
5-Dehydro-4-deoxy-D-glucarate
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
-
inhibition after prolonged incubation
iodoacetate
-
inhibition after prolonged incubation
p-chloromercuribenzene sulfonic acid
-
-
Sodium borohydride
-
95% inhibition only when 5-dehydro-4-deoxy-D-glucarate is added
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.203
5-dehydro-4-deoxy-D-glucarate
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.9
5-dehydro-4-deoxy-D-glucarate
Acinetobacter baylyi
Q6FFQ1
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
sharp decrease in activity below pH 6.5 and above pH 8
PDB
SCOP
CATH
ORGANISM
UNIPROT
Agrobacterium fabrum (strain C58 / ATCC 33970)
Agrobacterium fabrum (strain C58 / ATCC 33970)
Agrobacterium fabrum (strain C58 / ATCC 33970)
Agrobacterium fabrum (strain C58 / ATCC 33970)
Agrobacterium fabrum (strain C58 / ATCC 33970)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30500
-
4 * 30500, SDS-PAGE
55000
determined by SDS-PAGE
113000
-
sedimentation equilibrium centrifugation
118000
-
sedimentation equilibrium
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 30500, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells
Show AA Sequence (1823 entries)
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