Information on EC 4.2.1.35 - (R)-2-Methylmalate dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.35
-
RECOMMENDED NAME
GeneOntology No.
(R)-2-Methylmalate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-Methylmalate = 2-methylmaleate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
addition
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2,5-xylenol and 3,5-xylenol degradation
-
-
C5-Branched dibasic acid metabolism
-
-
L-isoleucine biosynthesis II
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-
Metabolic pathways
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Valine, leucine and isoleucine biosynthesis
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-
isoleucine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
(R)-2-methylmalate hydro-lyase (2-methylmaleate-forming)
Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-92-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Methylmaleate + H2O
(R)-2-Methylmalate
show the reaction diagram
2-Methylmaleate + H2O
?
show the reaction diagram
-
enzyme in the pathway of metabolism of 3,5-xylenol and oxidation of p-cresol
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-Methylmaleate + H2O
?
show the reaction diagram
-
enzyme in the pathway of metabolism of 3,5-xylenol and oxidation of p-cresol
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
required for maximal activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
-
1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH
diphosphate
-
1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH
EDTA
-
1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH
iodoacetate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cys
-
sulfhydryl compound is required for maximal activity
GSH
-
sulfhydryl compound is required for maximal activity
mercaptoethanol
-
sulfhydryl compound is required for maximal activity
Thioglycollate
-
sulfhydryl compound is required for maximal activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
2-methylmaleate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 8.2
-
pH 6.3: about 30% of maximal activity, pH 8.2: about 55% of maximal activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE