Information on EC 4.2.1.27 - acetylenecarboxylate hydratase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY
4.2.1.27
-
RECOMMENDED NAME
GeneOntology No.
acetylenecarboxylate hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-oxopropanoate = propynoate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
addition
-
-
-
-
hydration
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-Alanine metabolism
-
-
Butanoate metabolism
-
-
Propanoate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3-oxopropanoate hydro-lyase (propynoate-forming)
The reaction is effectively irreversible, favouring oxopropanoate (malonic semialdehyde) and its tautomers. Also acts on but-3-ynoate forming acetoacetate. The mechanism appears to involve hydration of the acetylene to 3-hydroxypropenoate, which will spontaneously tautomerize to 3-oxopropanoate. It is thus analogous to EC 4.1.1.78, acetylenedicarboxylate decarboxylase, in its mechanism.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acetylenemonocarboxylic acid hydrase
-
-
-
-
dehydratase, malonate semialdehyde
-
-
-
-
EC 4.2.1.71
-
-
formerly
-
malonate semialdehyde dehydratase
-
-
-
-
malonate semialdehyde dehydratase, malonate semialdehyde
-
-
-
-
malonate-semialdehyde dehydratase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9024-26-4
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
propynoate + H2O
3-oxopropanoate
show the reaction diagram
-
i.e. acetylenemonocarboxylate
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
diethanolamine
-
-
ethanolamine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
40.08
propynoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.4 - 8.8
-
pH 6.4: about 55% of maximal activity, pH 8.8: about 35% of maximal activity
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7
-
3°C, 18 h, enzyme in dilute solution, 18 h, 20% loss of activity
644460
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, stable at
-
3°C, pH 7-8, 18 h, enzyme in dilute solution, 18 h, 20% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE