Information on EC 4.2.1.27 - acetylenecarboxylate hydratase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY
4.2.1.27
-
RECOMMENDED NAME
GeneOntology No.
acetylenecarboxylate hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3-oxopropanoate = propynoate + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
addition
-
-
-
-
hydration
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
beta-Alanine metabolism
-
Butanoate metabolism
-
Propanoate metabolism
-
SYSTEMATIC NAME
IUBMB Comments
3-oxopropanoate hydro-lyase (propynoate-forming)
The reaction is effectively irreversible, favouring oxopropanoate (malonic semialdehyde) and its tautomers. Also acts on but-3-ynoate forming acetoacetate. The mechanism appears to involve hydration of the acetylene to 3-hydroxypropenoate, which will spontaneously tautomerize to 3-oxopropanoate. It is thus analogous to EC 4.1.1.78, acetylenedicarboxylate decarboxylase, in its mechanism.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acetylenemonocarboxylic acid hydrase
-
-
-
-
dehydratase, malonate semialdehyde
-
-
-
-
EC 4.2.1.71
-
-
formerly
-
malonate semialdehyde dehydratase
-
-
-
-
malonate semialdehyde dehydratase, malonate semialdehyde
-
-
-
-
malonate-semialdehyde dehydratase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9024-26-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
propynoate + H2O
3-oxopropanoate
show the reaction diagram
-
i.e. acetylenemonocarboxylate
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
diethanolamine
-
-
ethanolamine
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
40.08
-
propynoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.4
8.8
-
pH 6.4: about 55% of maximal activity, pH 8.8: about 35% of maximal activity
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
3°C, 18 h, enzyme in dilute solution, 18 h, 20% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-15°C, stable at
-
3°C, pH 7-8, 18 h, enzyme in dilute solution, 18 h, 20% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE