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Information on EC 4.2.1.2 - fumarate hydratase and Organism(s) Saccharolobus solfataricus and UniProt Accession P39461

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.2 fumarate hydratase
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This record set is specific for:
Saccharolobus solfataricus
UNIPROT: P39461 not found.
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Word Map
The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
fumarase, fumarate hydratase, fumarase c, class ii fumarase, lmfh-2, scfumc, slfumc, mmcbc, lmfh-1, stfumc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
fumarase
-
-
-
-
hydratase, fumarate
-
-
-
-
L-malate hydro-lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-malate hydro-lyase (fumarate-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9032-88-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
fumarate + H2O
L-malate
show the reaction diagram
-
-
-
r
(S)-malate
fumarate + H2O
show the reaction diagram
-
-
-
r
fumarate + H2O
L-malate
show the reaction diagram
-
-
-
r
additional information
?
-
-
no activity with D-malate, maleate, citramalate and citrate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-propanol
-
15-20% of maximum activity when assayed in 40% (v/v) 2-propanol
ethanol
-
15-20% of maximum activity when assayed in 40% (v/v) ethanol
methanol
-
94% of maximum activity when assayed in 40% (v/v) methanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
(S)-malate
0.125
fumarate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
pH 5.5: 43% of maximal activity, pH 9.0: 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85 - 95
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47900
calculated from amino acid sequence
160000
-
sucrose density gradient centrifugation
170000
-
gel filtration
45000
-
4 * 45000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 45000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
30 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% v/v ethanol, 50% v/v methanol, 50% v/v 2-propanol, 4 M urea or 0.1% SDS, stable after treatment for 10 h at room temperature
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-propanol
-
50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days
Ethanol
-
50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days
Methanol
-
50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days
SDS
-
0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days
urea
-
0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain JM109
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Puchegger, S.; Redl, B.; Stpfler, G.
Purification and properties of a thermostable fumarate hydratase from the archaeobacterium Sulfolobus solfataricus
J. Gen. Microbiol.
136
1537-1541
1990
Saccharolobus solfataricus, Saccharolobus solfataricus DSM 1616
Manually annotated by BRENDA team
Colombo, S.; Grisa, M.; Tortora, P.; Vanoni, M.
Molecular cloning, nucleotide sequence and expression of a Sulfolobus solfataricus gene encoding a class II fumarase
FEBS Lett.
337
93-98
1994
Saccharolobus solfataricus (P39461), Saccharolobus solfataricus, Saccharolobus solfataricus MT-4 / DSM 5833 (P39461)
Manually annotated by BRENDA team