Information on EC 4.2.1.19 - imidazoleglycerol-phosphate dehydratase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.2.1.19
-
RECOMMENDED NAME
GeneOntology No.
imidazoleglycerol-phosphate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
histidine metabolism
-
-
Histidine metabolism
-
-
L-histidine biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
D-erythro-1-(imidazol-4-yl)glycerol-3-phosphate hydro-lyase [3-(imidazol-4-yl)-2-oxopropyl-phosphate-forming]
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CAS REGISTRY NUMBER
COMMENTARY hide
9024-35-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cabbage
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Candida humilis CBS 8195
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
barley
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
rice
-
-
Manually annotated by BRENDA team
rose
-
-
Manually annotated by BRENDA team
wheat
-
-
Manually annotated by BRENDA team
corn
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
show the reaction diagram
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
sixth step in histidine biosynthesis
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
show the reaction diagram
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
P0CO22
sixth step in histidine biosynthesis
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
metalloprotein, transition metals induce aggregation and are required for catalysis, 1 equivalent of Mn2+ per subunit induces the formation of 24-mers from the trimeric enzyme
Mn
-
the manganese cluster is critical in converting the inactive trimeric state of the enzyme into its biologically active 24-mer and also forms the active site. The substrate is bound to the manganese cluster as an imidazole moiety that subsequently collapse to yield a diazafulvene intermediate
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-1,2,4-triazole
phosphate
[3-Hydroxy-2-(1H-[1,2,4]triazol-3-yl)-butyl]-phosphonic acid
-
IRL 1803
[3-Hydroxy-2-(1H-[1,2,4]triazol-3-yl)-cyclohexyl]-phosphonic acid
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IRL 1856
[3-Hydroxy-2-(1H-[1,2,4]triazol-3-yl)-propyl]-phosphonic acid
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IRL 1695
additional information
-
not inhibited by histidinol phosphate, histidinol, histidine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
cysteine
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activation
mercaptoethylamine
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.049 - 0.54
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1000
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
Mycobacterium tuberculosis
-
at pH 7.7 and 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03 - 0.31
3-Amino-1,2,4-triazole
0.008
phosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
-
8
-
enzyme expressed in Escherichia coli
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 8
-
wild-type enzyme, less than 50% of maximal activity above and below
7.2 - 8.2
-
enzyme expressed in E. coli, less than 50% of maximal activity above and below
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
trimeric enzyme in absence of Mn2+, gel filtration
260000
-
bifunctional enzyme with EC 3.1.3.15, gel filtration
290000
-
gel filtration
300000
-
gel electrophoresis
500000
-
polymeric enzyme in presence of Mn2+, gel filtration
540000
-
gel filtration
570000
-
gel filtration
600000
-
gel filtration
670000
-
gel electrophoresis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
multimer
1 equivalent of Mn2+ per subunit induces the formation of 24-mers from the trimeric enzyme
polymer
tetradodecamer
-
-
trimer
1 equivalent of Mn2+ per subunit induces the formation of 24-mers from the trimeric enzyme
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallized in presence of Mn, unit-cell parameters: a = b = 157.9 A, c = 480 A, alpha = beta = 90, gamma = 120, with either 16 or 24 subunits in the asymmetric unit, space group R3, 3.0 A resolution
-
crystals belong to space group R3 with cell parameters a = 157.9 A, b = 157.9 A, c = 480 A, alpha = beta = 90, gamma = 120. Structure of manganese assembled, active form of the enzyme at 3.0 A resolution
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trimeric form without Mn2+, hanging drop vapor diffusion method
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
irreversible inactivation below
5531
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 20 mM triethanolamine-HCl buffer, pH 7.5, 100 mM 2-mercaptoethanol
-
Mg2+, Mn2+, glycerol, dithiothreitol do not stablilize
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
photooxidation in presence of Rose Bengal
-
5534
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Mycobacterium smegmatis strain mc24517
-
expressed in Saccharomyces cerevisiae strain BY4742
expression in Escherichia coli
-
expression in Escherichia coli BL21
-
expression in Saccharomyces cerevisiae
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