Information on EC 4.2.1.154 - tetracenomycin F2 cyclase

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The expected taxonomic range for this enzyme is: Streptomyces glaucescens

EC NUMBER
COMMENTARY hide
4.2.1.154
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RECOMMENDED NAME
GeneOntology No.
tetracenomycin F2 cyclase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
tetracenomycin F2 = tetracenomycin F1 + H2O
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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Biosynthesis of type II polyketide products
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elloramycin biosynthesis
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tetracenomycin C biosynthesis
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elloramycin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
tetracenomycin F2 hydro-lyase (tetracenomycin-F1-forming)
The enzyme is involved in biosynthesis of the anthracycline antibiotic tetracenomycin C by the bacterium Streptomyces glaucescens.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tetracenomycin F2
tetracenomycin F1 + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tetracenomycin F2
tetracenomycin F1 + H2O
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.121 - 1.2
tetracenomycin F2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0146 - 0.142
tetracenomycin F2
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0121 - 0.916
tetracenomycin F2
193508
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000571
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cell-free extract, at pH 8.0 and 30°C
0.309
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after 541fold purification, at pH 8.0 and 30°C
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12500
-
3 * 12500, SDS-PAGE
12728
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3 * 12728, calculated from amino acid sequence
37500
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotrimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
macroseeding method, using 1.4 M (NH4)2SO4, 50 mM HEPES, pH 7.5
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Mono Q column chromatography, phenyl-Superose gel filtration, Superose 6 gel filtration, and Sephacryl S-200 gel filtration
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Q-Sepharose column chromatography, phenyl-Sepharose column chromatography, and MonoQ column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D27N
the mutant shows 14% activity compared to the wild type enzyme
H26A
the mutant shows 15% activity compared to the wild type enzyme
H26Q
the mutant shows 91% activity compared to the wild type enzyme
H51A
the mutant shows 16% activity compared to the wild type enzyme
H51Q
the mutant shows 96% activity compared to the wild type enzyme
R40G
the mutant shows 10% activity compared to the wild type enzyme
R40K
the mutant shows 16% activity compared to the wild type enzyme