Information on EC 4.2.1.147 - 5,6,7,8-tetrahydromethanopterin hydro-lyase

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The expected taxonomic range for this enzyme is: Methylobacterium extorquens

EC NUMBER
COMMENTARY hide
4.2.1.147
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RECOMMENDED NAME
GeneOntology No.
5,6,7,8-tetrahydromethanopterin hydro-lyase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
formaldehyde oxidation V (H4MPT pathway)
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Methane metabolism
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Microbial metabolism in diverse environments
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methanogenesis from CO2
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SYSTEMATIC NAME
IUBMB Comments
5,6,7,8-tetrahydromethanopterin hydro-lyase (formaldehyde-adding, tetrahydromethanopterin-forming)
Found in methylotrophic bacteria and methanogenic archaea.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,6,7,8-tetrahydromethanopterin + formaldehyde
5,10-methylenetetrahydromethanopterin + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tetrahydromethanopterin
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dependent on
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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stimulates activity, Ka: 4 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
5,6,7,8-tetrahydromethanopterin
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30°C, pH 8.0
0.2
formaldehyde
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30°C, pH 8.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20.2
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30°C, pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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one of the major proteins in the cytoplasm
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
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3 * 18000, SDS-PAGE
60000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homopentamer
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x-ray crystallography
homotrimer
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3 * 18000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
non-labeled and selenomethionine-labeled enzyme, hanging drop vapor diffusion method, using 0.2 M calcium chloride hydrate, 0.1 M sodium acetate hydrate, pH 4.6, and 10-20% (v/v) isopropanol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) pLysS cells
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Show AA Sequence (178 entries)
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