Information on EC 4.2.1.143 - versicolorin B synthase

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The expected taxonomic range for this enzyme is: Aspergillus parasiticus

EC NUMBER
COMMENTARY hide
4.2.1.143
-
RECOMMENDED NAME
GeneOntology No.
versicolorin B synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
versiconal = versicolorin B + H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aflatoxin biosynthesis
-
-
versicolorin B biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
versiconal hydro-lyase (versicolorin-B forming)
Isolated from the aflatoxin-producing mold Aspergillus parasiticus. Involved in aflatoxin biosynthesis.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
versiconal
(-)-versicolorin B + H2O
show the reaction diagram
versiconal
versicolorin B + H2O
show the reaction diagram
versiconal hemiacetal
versicolorin B + H2O
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactors required
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-ethyl-3(3-dimethylaminopropyl)carbodiimide
-
10 mM, 12% residual activity
3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol
diethyl dicarbonate
-
10 mM, 41% residual activity
Hg2+
-
1 mM, complete loss of activity
versicolorin B
additional information
-
not inhibitory: FAD, FMN, riboflavin, and glucose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0031 - 0.0065
versiconal
0.0024
versiconal hemiacetal
-
pH 6.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
versiconal
Aspergillus parasiticus
-
pH 6.5, 37°C
2.5
versiconal hemiacetal
Aspergillus parasiticus
-
pH 6.5, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.076 - 0.096
3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1900
-
pH 6.5, 37°C
2750
-
pH 6.5, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
drastic decline in activity below
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoelectric focusing
4.6
-
isoelectric focusing
4.7
-
isoelectric focusing
5.1
-
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest abundance of enzyme in colony fraction S2 consisting of cells that have grown for 24-48 h
Manually annotated by BRENDA team
-
asexual developmental structures
Manually annotated by BRENDA team
-
vegetative hyphae
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
enzyme distributes throughout the cytoplasm and concentrates in ring-like structures surrounding nuclei
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein
70271
-
x * 70271, calculated, x * 78000, SDS-PAGE
135000
-
gel filtration
140000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Pichia pastoris
expression in Saccharomyces cerevisiae
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information