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Information on EC 4.2.1.142 - 5'-oxoaverantin cyclase and Organism(s) Aspergillus parasiticus and UniProt Accession Q12062

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.142 5'-oxoaverantin cyclase
IUBMB Comments
Isolated from the aflatoxin-producing mold Aspergillus parasiticus. The enzyme also catalyses the conversion of versiconal to versicolorin B (EC 4.2.1.143, versicolorin B synthase). Involved in aflatoxin biosynthesis.
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This record set is specific for:
Aspergillus parasiticus
UNIPROT: Q12062
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The taxonomic range for the selected organisms is: Aspergillus parasiticus
The enzyme appears in selected viruses and cellular organisms
Synonyms
oavn cyclase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
versicolorin B synthase
-
OAVN cyclase
SYSTEMATIC NAME
IUBMB Comments
5'-oxoaverantin hydro-lyase [(2'S,5'S)-averufin forming]
Isolated from the aflatoxin-producing mold Aspergillus parasiticus. The enzyme also catalyses the conversion of versiconal to versicolorin B (EC 4.2.1.143, versicolorin B synthase). Involved in aflatoxin biosynthesis.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-oxoaverantin
(2'S,5'S)-averufin + H2O
show the reaction diagram
i.e. 1,3,6,8-tetrahydroxy-2-[(1S)-1-hydroxy-5-oxohexyl]anthracene-9,10-dione
i.e. 7,9,11-trihydroxy-2-methyl-3,4,5,6-tetrahydro-2,6-epoxy-2H-anthra[2,3-b]oxocin-8,13-dione
-
?
5'-oxoaverantin
(2'S,5'S)-averufin + H2O
show the reaction diagram
-
i.e. 1,3,6,8-tetrahydroxy-2-[(1S)-1-hydroxy-5-oxohexyl]anthracene-9,10-dione
i.e. 7,9,11-trihydroxy-2-methyl-3,4,5,6-tetrahydro-2,6-epoxy-2H-anthra[2,3-b]oxocin-8,13-dione
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no requirement for NAD(H) or NADP(H)
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
5'-oxoaverantin
-
pH 7.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0836
-
pH 7.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme is involved in aflatoxin biosynthesis. A deletion mutant accumulates large amounts of 5'-oxoaverantin, 5'-hydroxyaverantin, averantin, (2'S,5'S)-averufin, and averufanin in the mycelium, leading to bright orange colour
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
158000
-
gel filtration
79000
-
2 * 79000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 79000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sakuno, E.; Yabe, K.; Nakajima, H.
Involvement of two cytosolic enzymes and a novel intermediate, 5-oxoaverantin, in the pathway from 5-hydroxyaverantin to averufin in aflatoxin biosynthesis
Appl. Environ. Microbiol.
69
6418-6426
2003
Aspergillus parasiticus
Manually annotated by BRENDA team
Sakuno, E.; Wen, Y.; Hatabayashi, H.; Arai, H.; Aoki, C.; Yabe, K.; Nakajima, H.
Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis
Appl. Environ. Microbiol.
71
2999-3006
2005
Aspergillus parasiticus (Q12062), Aspergillus parasiticus, Aspergillus parasiticus NRRL 2999 (Q12062)
Manually annotated by BRENDA team