Information on EC 4.2.1.138 - (+)-caryolan-1-ol synthase

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The expected taxonomic range for this enzyme is: Streptomyces griseus

EC NUMBER
COMMENTARY hide
4.2.1.138
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RECOMMENDED NAME
GeneOntology No.
(+)-caryolan-1-ol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-beta-caryophyllene + H2O = (+)-caryolan-1-ol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(+)-beta-caryophyllene hydrolase [cyclizing, (+)-caryolan-1-ol-forming]
A multifunctional enzyme which also forms (+)-beta-caryophyllene from farnesyl diphosphate [EC 4.2.3.89, (+)-beta-caryophyllene synthase].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-beta-caryophyllene + H2O
(+)-caryolan-1-ol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2
Mn2+
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38% activity (relative to the activity in the presence ofMg2+) is retained in the presence of 5 mM Mn2+
additional information
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no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
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x * 41000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells
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