Information on EC 4.2.1.134 - very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
4.2.1.134
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RECOMMENDED NAME
GeneOntology No.
very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoate biosynthesis (6-desaturase)
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(4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoate biosynthesis II (4-desaturase)
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arachidonate biosynthesis I (6-desaturase, lower eukaryotes)
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arachidonate biosynthesis III (6-desaturase, mammals)
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arachidonate biosynthesis IV (8-detaturase, lower eukaryotes)
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arachidonate biosynthesis V (8-detaturase, mammals)
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Biosynthesis of secondary metabolites
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Biosynthesis of unsaturated fatty acids
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docosahexaenoate biosynthesis I (lower eukaryotes)
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docosahexaenoate biosynthesis III (6-desaturase, mammals)
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docosahexaenoate biosynthesis IV (4-desaturase, mammals)
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Fatty acid elongation
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hydroxylated fatty acid biosynthesis (plants)
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icosapentaenoate biosynthesis I (lower eukaryotes)
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icosapentaenoate biosynthesis II (6-desaturase, mammals)
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icosapentaenoate biosynthesis III (8-desaturase, mammals)
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icosapentaenoate biosynthesis V (8-desaturase, lower eukaryotes)
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juniperonate biosynthesis
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sciadonate biosynthesis
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very long chain fatty acid biosynthesis I
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very long chain fatty acid biosynthesis II
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SYSTEMATIC NAME
IUBMB Comments
very-long-chain (3R)-3-hydroxyacyl-CoA hydro-lyase
This is the third component of the elongase, a microsomal protein complex responsible for extending palmitoyl-CoA and stearoyl-CoA (and modified forms thereof) to very-long chain acyl CoAs. cf. EC 2.3.1.199, very-long-chain 3-oxoacyl-CoA synthase, EC 1.1.1.330, very-long-chain 3-oxoacyl-CoA reductase, and EC 1.3.1.93, very-long-chain enoyl-CoA reductase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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the enzyme catalyzes the third reaction of the four-step cycle in the elongation of very long-chain fatty acids
physiological function
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the enzyme is essential and limiting for Arabidopsis development. Enzyme-derived very-long-chain fatty acid homeostasis is required for specific developmental processes. The enzyme is involved in 3-hydroxy acyl-coa dehydration during very-long-chain fatty acid elongation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxyacyl-CoA
2,3-trans-enoyl-CoA + H2O
show the reaction diagram
3-hydroxyacyl-CoA
enoyl-CoA + H2O
show the reaction diagram
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?
3-hydroxypalmitoyl-CoA
2,3-trans-hexadecenoyl-CoA + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxyacyl-CoA
2,3-trans-enoyl-CoA + H2O
show the reaction diagram
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0068 - 0.1217
3-hydroxypalmitoyl-CoA
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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a membranespanning protein that traverses the membrane six times and has an N-terminus and C-terminus facing the cytosol
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence; x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence; x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence; x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana and Arabidopsis thaliana epidermal cells
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expressed in Saccharomyces cerevisiae strain SAY32
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E116A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
E156A
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inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally
E60A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
F145A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
G152A
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mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally
L12A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
N16A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
P150A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
P188A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
Q200A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
Q54A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
Q79A
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inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally
R119A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
R141A
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mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally
R201A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
R83A
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mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally
W124A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
W23A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
Y142A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells
Y149A
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inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally
Y15A
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phytosphingosine accumulates at low levels in the mutant compared with wild type cells