Information on EC 4.2.1.131 - carotenoid 1,2-hydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.131
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RECOMMENDED NAME
GeneOntology No.
carotenoid 1,2-hydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,1'-dihydroxy-1,1',2,2'-tetrahydrolycopene = 1-hydroxy-1,2-dihydrolycopene + H2O
show the reaction diagram
(2)
-
-
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1-hydroxy-1,2-dihydrolycopene = lycopene + H2O
show the reaction diagram
(1)
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Carotenoid biosynthesis
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Metabolic pathways
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myxol-2' fucoside biosynthesis
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spheroidene and spheroidenone biosynthesis
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spirilloxanthin and 2,2'-diketo-spirilloxanthin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
lycopene hydro-lyase (1-hydroxy-1,2-dihydrolycopene-forming)
In Rubrivivax gelatinosus [1] and Thiocapsa roseopersicina [2] both products are formed, whereas Rhodobacter capsulatus [1] only gives 1-hydroxy-1,2-dihydrolycopene. Also acts on neurosporene giving 1-hydroxy-1,2-dihydroneurosporene with both organism but 1,1'-dihydroxy-1,1',2,2'-tetrahydroneurosporene only with Rubrivivax gelatinosus.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-hydroxy-3,4-didehydrolycopene + H2O
?
show the reaction diagram
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conversion rate 20%
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-
?
1-hydroxylycopene + H2O
1,1'-dihydroxylycopene
show the reaction diagram
1-hydroxyneurosporene + H2O
1,1'-dihydroxyneurosporene
show the reaction diagram
demethylspheroidene + H2O
?
show the reaction diagram
-
conversion rate 14%
-
-
?
gamma-carotene + H2O
1'-hydroxy-gamma-carotene
show the reaction diagram
geranylgeraniol + H2O
?
show the reaction diagram
lycopene + H2O
1,1'-dihydroxylycopene
show the reaction diagram
lycopene + H2O
1-hydroxylycopene
show the reaction diagram
neurosporene + H2O
1-hydroxyneurosporene
show the reaction diagram
spheroidene + H2O
?
show the reaction diagram
-
conversion rate 12%
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0368
1-hydroxyneurosporene
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pH 7.8, 28°C
0.0095 - 0.0269
lycopene
0.0245 - 0.0385
Neurosporene
0.0078
spheroidene
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pH 7.8, 28°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16 - 0.19
lycopene
2854
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
50% residual activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 35
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
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x * 38000, SDS-PAGE
40000
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x * 40000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli