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Information on EC 4.2.1.128 - lupan-3beta,20-diol synthase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9C5M3

for references in articles please use BRENDA:EC4.2.1.128
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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.128 lupan-3beta,20-diol synthase
IUBMB Comments
The reaction occurs in the reverse direction. The recombinant enzyme from Arabidopsis thaliana gives a 1:1 mixture of lupeol and lupan-3beta,20-diol with small amounts of beta-amyrin, germanicol, taraxasterol and psi-taraxasterol. See EC 5.4.99.41 (lupeol synthase).
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Arabidopsis thaliana
UNIPROT: Q9C5M3
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Arabidopsis thaliana
Synonyms
At1g78970, LUP1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
(3S)-2,3-epoxy-2,3-dihydrosqualene hydro-lyase (lupan-3beta,20-diol forming)
The reaction occurs in the reverse direction. The recombinant enzyme from Arabidopsis thaliana gives a 1:1 mixture of lupeol and lupan-3beta,20-diol with small amounts of beta-amyrin, germanicol, taraxasterol and psi-taraxasterol. See EC 5.4.99.41 (lupeol synthase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
lupan-3beta,20-diol
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
lupan-3beta,20-diol
show the reaction diagram
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-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LUP1_ARATH
757
0
87352
Swiss-Prot
other Location (Reliability: 2)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Segura, M.J.; Meyer, M.M.; Matsuda, S.P.
Arabidopsis thaliana LUP1 converts oxidosqualene to multiple triterpene alcohols and a triterpene diol
Org. Lett.
2
2257-2259
2000
Arabidopsis thaliana (Q9C5M3)
Manually annotated by BRENDA team
Kushiro, T.; Hoshino, M.; Tsutsumi, T.; Kawai, K.i.; Shiro, M.; Shibuya, M.; Ebizuka, Y.
Stereochemical course in water addition during LUP1-catalyzed triterpene cyclization
Org. Lett.
8
5589-5592
2006
Arabidopsis thaliana (Q9C5M3)
Manually annotated by BRENDA team