Information on EC 4.2.1.117 - 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)

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The expected taxonomic range for this enzyme is: Shewanella oneidensis

EC NUMBER
COMMENTARY hide
4.2.1.117
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RECOMMENDED NAME
GeneOntology No.
2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S,3S)-2-methylcitrate = 2-methyl-trans-aconitate + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-methylcitrate cycle II
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Propanoate metabolism
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SYSTEMATIC NAME
IUBMB Comments
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase (2-methyl-trans-aconitate forming)
Catalyses the dehydration of (2S,3S)-2-methylcitrate, forming the trans isomer of 2-methyl-aconitate (unlike EC 4.2.1.79, which forms only the cis isomer). Part of a propionate degradation pathway. The enzyme from Shewanella oneidensis can also accept citrate and cis-aconitate, but activity with (2S,3S)-2-methylcitrate was approximately 2.5-fold higher. 2-methylisocitrate and isocitrate were not substrates [1]. An iron-sulfur protein.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methylcitrate
2-methyl-cis-aconitate + H2O
show the reaction diagram
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-
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?
cis-aconitate + H2O
citrate
show the reaction diagram
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?
citrate
cis-aconitate + H2O
show the reaction diagram
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r
additional information
?
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no substrate: 2-methylisocitrate, isocitrate. Enzyme only catalyzes the first half of the aconitase-like reaction
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4Fe-4S-center
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enzyme requires an intact Fe/S cluster for activity
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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enzyme requires an intact Fe/S cluster for activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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complete loss of activity
ferricyanide
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complete loss of activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.9
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pH 8.0, 37°C, substrate citrate
5
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pH 8.0, 37°C, substrate cis-aconitate
7.8
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pH 8.0, 37°C, substrate 2-methylcitrate
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
94000
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x * 94000, SDS-PAGE and calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 94000, SDS-PAGE and calculated
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
in presence of air, activity is lost over time
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651735
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE