Information on EC 4.2.1.111 - 1,5-anhydro-D-fructose dehydratase

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The expected taxonomic range for this enzyme is: Pezizaceae

EC NUMBER
COMMENTARY
4.2.1.111
-
RECOMMENDED NAME
GeneOntology No.
1,5-anhydro-D-fructose dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
1,5-anhydro-D-fructose = 1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
show the reaction diagram
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
NIL
-
SYSTEMATIC NAME
IUBMB Comments
1,5-anhydro-D-fructose hydro-lyase (ascopyrone-M-forming)
This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. The other enzymes involved in this pathway are EC 4.2.1.110 (aldos-2-ulose dehydratase), EC 4.2.2.13 [exo-(1->4)-alpha-D-glucan lyase] and EC 5.3.2.7 (ascopyrone tautomerase). Requires divalent (Ca2+ or Mg2+) or monovalent cations (Na+) for optimal activity. Unlike EC 4.2.1.110, the enzyme is specific for 1,5-anhydro-D-fructose as substrate and shows no activity towards aldose-2-uloses such as 2-dehydroglucose [1,2,3]. In addition, it is inhibited by its end-product ascopyrone M [2] and it cannot convert ascopyrone M into microthecin, as can EC 4.2.1.110.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,5-anhydro-D-arabino-hex-2-ulose dehydratase
Anthracobia melaloma
-
-
1,5-anhydro-D-arabino-hex-2-ulose dehydratase
Anthracobia melaloma CBS 293.54
-
-
-
1,5-anhydro-D-fructose 4-dehydratase
Anthracobia melaloma
-
-
1,5-anhydro-D-fructose 4-dehydratase
Anthracobia melaloma CBS 293.54
-
-
-
1,5-anhydro-D-fructose hydro-lyase
Anthracobia melaloma
-
-
1,5-anhydro-D-fructose hydrolyase
Anthracobia melaloma
-
-
1,5-anhydro-D-fructose hydrolyase
Anthracobia melaloma CBS 293.54
-
-
-
AF dehydratase
Anthracobia melaloma
-
-
AF dehydratase
Anthracobia melaloma CBS 293.54
-
-
-
AFDH
Anthracobia melaloma
-
-
AFDH
Anthracobia melaloma CBS 293.54
-
-
-
AFDH
Anthracobia melanoma, Peziza petersii
-
-
CAS REGISTRY NUMBER
COMMENTARY
9044-86-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Anthracobia melaloma
-
-
-
Manually annotated by BRENDA team
Anthracobia melanoma
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-fructose
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
show the reaction diagram
Anthracobia melaloma
-
i.e. 1,5-anhydro-D-arabino-hex-2-ulose. This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose, i.e. 1,5-anhydro-D-arabino-hex-2-ulose
i.e.ascopyrone M
-
ir
1,5-anhydro-D-fructose
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
show the reaction diagram
-
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
i.e.ascopyrone M
-
ir
1,5-anhydro-D-fructose
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
show the reaction diagram
-
AFDH is highly specific towards 1,5-anhydro-D-fructose
-
-
?
additional information
?
-
Anthracobia melaloma
-
the enzyme is inactive towards: glucosone, glucose, galactose, mannose, fructose, ribose and gamma-gluconolactone
-
-
-
additional information
?
-
-
AFDH is inactive towards D-glucose, D-galactose, D-mannose, D-fructose and D-ribose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-fructose
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
show the reaction diagram
Anthracobia melaloma
-
i.e. 1,5-anhydro-D-arabino-hex-2-ulose. This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose
i.e.ascopyrone M
-
ir
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
Anthracobia melanoma
-
the activity increases by around 2fold in the presence of 10 mM CaCl2
CaCl2
Anthracobia melaloma
-
requirement of alkaline earth metal ion for activity. 10 mM, around 2fold increase in activity
Mg2+
Anthracobia melanoma
-
the activity increases by around 2fold in the presence of 25 mM MgCl2
MgCl2
Anthracobia melaloma
-
requirement of alkaline earth metal ion for activity. 25 mM, around 2fold increase in activity
Na+
Anthracobia melanoma
-
the activity increases by around 2fold in the presence of 0.1 M NaCl
NaCl
Anthracobia melaloma
-
0.1 mM, around 2fold increase in activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
Anthracobia melaloma
-
50 mM, 84% inhibition
EDTA
Anthracobia melanoma
-
50 mM of EDTA inhibits AFDH activity by 84%
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1870
-
Anthracobia melaloma
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
Anthracobia melaloma
-
optimal pH-range is 5.9-7.0
6.5
-
Anthracobia melanoma
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.9
7
Anthracobia melaloma
-
optimal pH-range
5.9
7
Anthracobia melanoma
-
-
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
140000
-
Anthracobia melaloma
-
gel filtration
230000
-
Anthracobia melaloma
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Anthracobia melaloma
-
x * 98000, SDS-PAGE
?
Anthracobia melaloma CBS 293.54
-
x * 98000, SDS-PAGE
-
dimer
Anthracobia melaloma
-
2 * 98000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Anthracobia melaloma
-