Information on EC 4.2.1.107 - 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Euarchontoglires

EC NUMBER
COMMENTARY
4.2.1.107
-
RECOMMENDED NAME
GeneOntology No.
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
elimination
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acid biosynthesis, neutral pathway
-
-
bile acid biosynthesis, neutral pathway
-
-
Metabolic pathways
-
-
Primary bile acid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]
This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the beta-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
46 kDa hydratase
-
-
46 kDa hydratase 2
-
-
perMFE-2
-
multifunctional enzyme, cf. EC 4.2.1.119, second multifunctional enzyme in rat liver peroxisome perMFE-1, cf. EC 1.1.1.35 and EC 5.3.3.8
CAS REGISTRY NUMBER
COMMENTARY
152787-68-3
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
product of the reaction (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA is characterized as a physiological intermediate in bile acid synthesis, in the beta-oxidative side-chain modifications
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-en-26-oyl-CoA + H2O
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA
show the reaction diagram
-
-
-
-
?
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-en-26-oyl-CoA + H2O
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA
show the reaction diagram
-
reaction in cholic acid synthesis is catalyzed by peroxisomal multifunctional enzyme type 2. Human perMFE-2 is induced by transcription factors AP2alpha and AP2gamma and attenuated by Sp1 binding to recognition sequence located within and -151/-142 region
-
-
?
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA
show the reaction diagram
-
-
-
-
?
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA
show the reaction diagram
-
the equilibrium lies in the direction of the dehydration
only the (24R,25R) diastomer as product, analyzed using reverse-phase HPLC
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O
3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-en-26-oyl-CoA + H2O
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA
show the reaction diagram
-
reaction in cholic acid synthesis is catalyzed by peroxisomal multifunctional enzyme type 2. Human perMFE-2 is induced by transcription factors AP2alpha and AP2gamma and attenuated by Sp1 binding to recognition sequence located within and -151/-142 region
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
48
-
recombinant enzyme, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
46000
-
SDS-PAGE
684952
46580
-
amino acid sequence analysis
684952
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
still active after 3 h of incubation in the enzyme assay
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, 13% yield, anion- and cation exchange chromatography, and gel filtration on Superdex 200 HR
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) plysS cells, for analysis the role of the hydratase in the beta-oxidation
-